PMID- 23723157
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20220321
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 10
IP  - 2
DP  - 2016 Feb
TI  - Behaviour of human mesenchymal stem cells on chemically synthesized HA-PCL 
      scaffolds for hard tissue regeneration.
PG  - E147-54
LID - 10.1002/term.1768 [doi]
AB  - Our goal was to characterize the response of human mesenchymal stem cells (hMSCs) 
      to a novel composite scaffold for bone tissue engineering. The 
      hydroxyapatite-polycaprolactone (HA-PCL) composite scaffolds were prepared by a 
      sol-gel method at room temperature and the scaffold morphology was investigated 
      by scanning electron microscopy (SEM)/energy-dispersive spectroscopy (EDS) to 
      validate the synthesis process. The response of two different lines of hMSCs, 
      bone-marrow-derived human mesenchymal stem cells (BMSCs) and dental pulp stem 
      cells (DPSCs) in terms of cell proliferation and differentiation into the 
      osteoblastic phenotype, was evaluated using Alamar blue assay, SEM, histology and 
      alkaline phosphatase activity. Our results indicate that tissue engineering by 
      means of composite HA-PCL scaffolds may represent a new therapeutic strategy to 
      repair craniofacial bone defects.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - D'Anto, Vincenzo
AU  - D'Anto V
AD  - Institute of Composite and Biomedical Materials (IMCB), National Research Council 
      of Italy (CNR), Naples, Italy.
AD  - Department of Neurosciences, Reproductive Sciences and Oral Sciences, University 
      of Naples 'Federico II', Italy.
AD  - Department of Pediatric Surgery and Transplantation, Bambino Gesu Children's 
      Hospital, Rome, Italy.
FAU - Raucci, Maria Grazia
AU  - Raucci MG
AD  - Institute of Composite and Biomedical Materials (IMCB), National Research Council 
      of Italy (CNR), Naples, Italy.
FAU - Guarino, Vincenzo
AU  - Guarino V
AD  - Institute of Composite and Biomedical Materials (IMCB), National Research Council 
      of Italy (CNR), Naples, Italy.
FAU - Martina, Stefano
AU  - Martina S
AD  - Department of Neurosciences, Reproductive Sciences and Oral Sciences, University 
      of Naples 'Federico II', Italy.
FAU - Valletta, Rosa
AU  - Valletta R
AD  - Department of Neurosciences, Reproductive Sciences and Oral Sciences, University 
      of Naples 'Federico II', Italy.
FAU - Ambrosio, Luigi
AU  - Ambrosio L
AD  - Institute of Composite and Biomedical Materials (IMCB), National Research Council 
      of Italy (CNR), Naples, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130531
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Osteonectin)
RN  - 0 (Polyesters)
RN  - 24980-41-4 (polycaprolactone)
RN  - 91D9GV0Z28 (Durapatite)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - 3T3 Cells
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Cell Death/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Dental Pulp/cytology
MH  - Durapatite/*pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Mesenchymal Stem Cells/*cytology/drug effects/ultrastructure
MH  - Mice
MH  - Osteonectin/metabolism
MH  - Polyesters/*pharmacology
MH  - Spectrometry, X-Ray Emission
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - bone defects
OT  - composite material
OT  - dental pulp stem cells
OT  - human mesenchymal stem cells
OT  - hydroxyapatite
OT  - polymer
OT  - scaffold
EDAT- 2013/06/01 06:00
MHDA- 2016/11/01 06:00
CRDT- 2013/06/01 06:00
PHST- 2012/10/08 00:00 [received]
PHST- 2013/03/13 00:00 [revised]
PHST- 2013/04/13 00:00 [accepted]
PHST- 2013/06/01 06:00 [entrez]
PHST- 2013/06/01 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
AID - 10.1002/term.1768 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2016 Feb;10(2):E147-54. doi: 10.1002/term.1768. Epub 2013 
      May 31.