PMID- 23726292
OWN - NLM
STAT- MEDLINE
DCOM- 20131113
LR  - 20220409
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 94
DP  - 2013 Aug
TI  - Electronic waste leachate-mediated DNA fragmentation and cell death by apoptosis 
      in mouse fibroblast (NIH/3T3) cell line.
PG  - 87-93
LID - S0147-6513(13)00175-9 [pii]
LID - 10.1016/j.ecoenv.2013.05.004 [doi]
AB  - This study investigated the apoptotic effect of electronic waste on fibroblast 
      cell line. Cells were treated with different concentrations of the leachate for 
      24h. Cell viability was detected by MTT 
      (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, nuclear 
      morphology of cells was explored by acridine orange (AO)/ethidium bromide (EB) 
      double staining, mitochondrial membrane potential was evaluated using JC-1 probe 
      while cell cycle analysis was conducted using flow cytometry. The oxidative 
      status was detected using DCFH-DA (dichlorofluorescin diacetate) probe and the 
      relationship between cell death and ROS (reactive oxygen species) was 
      investigated using N-acetylcysteine. Results showed an increased cell death as 
      detected by MTT assay and AO/EB staining. Cell cycle analysis indicated an 
      induction of sub/G1 events while JC-1 probe showed significant disruption of 
      mitochondrial membrane potential. There was significant induction of ROS, while 
      N-acetylcysteine protected the cells from DNA damage. These suggest apoptotic 
      pathway as a possible mechanism of e-waste induced cyto-genotoxicity.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Alabi, Okunola A
AU  - Alabi OA
AD  - Department of Biosciences and Biotechnology, Babcock University, Ilisan Remo, 
      Ogun State, Nigeria.
FAU - Bakare, Adekunle A
AU  - Bakare AA
FAU - Filippin-Monteiro, Fabiola B
AU  - Filippin-Monteiro FB
FAU - Sierra, Jelver A
AU  - Sierra JA
FAU - Creczynski-Pasa, Tania B
AU  - Creczynski-Pasa TB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130528
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cell Cycle/drug effects
MH  - Cell Death/drug effects
MH  - Cell Survival/drug effects
MH  - DNA Fragmentation
MH  - *Electronic Waste
MH  - Fibroblasts/*drug effects
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Reactive Oxygen Species/metabolism
MH  - Risk Assessment
MH  - United States
MH  - Water Pollutants, Chemical/*toxicity
EDAT- 2013/06/04 06:00
MHDA- 2013/11/14 06:00
CRDT- 2013/06/04 06:00
PHST- 2013/03/26 00:00 [received]
PHST- 2013/05/03 00:00 [revised]
PHST- 2013/05/06 00:00 [accepted]
PHST- 2013/06/04 06:00 [entrez]
PHST- 2013/06/04 06:00 [pubmed]
PHST- 2013/11/14 06:00 [medline]
AID - S0147-6513(13)00175-9 [pii]
AID - 10.1016/j.ecoenv.2013.05.004 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2013 Aug;94:87-93. doi: 10.1016/j.ecoenv.2013.05.004. 
      Epub 2013 May 28.