PMID- 23726966
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20171116
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 132
IP  - 1
DP  - 2013 Jul
TI  - Down-regulation of endothelial protein C receptor shedding by persicarin and 
      isorhamnetin-3-O-galactoside.
PG  - e58-63
LID - S0049-3848(13)00196-5 [pii]
LID - 10.1016/j.thromres.2013.05.004 [doi]
AB  - Increasing evidence has shown that beyond its role in coagulation, endothelial 
      protein C receptor (EPCR) plays an important role in the cytoprotective pathway. 
      Previous reports have shown that EPCR can be shed from the cell surface, and that 
      this is mediated by tumor necrosis factor-alpha converting enzyme (TACE) and that 
      sEPCR levels are increased in patients with systemic inflammatory diseases. 
      Persicarin and isorhamnetin-3-O-galactoside (I3G) are active compounds from 
      Oenanthe javanica, which has been widely studied for its neuroprotective, 
      antioxidant, and barrier protective activities. However, little is known of the 
      effects of persicarin on EPCR shedding. Here, we investigated this issue by 
      monitoring the effects of persicarin and I3G on phorbol-12-myristate 13-acetate 
      (PMA) and on cecal ligation and puncture (CLP)-mediated EPCR shedding and 
      underlying mechanisms. According to the results, persicarin and I3G induced 
      potent inhibition of PMA and CLP-induced EPCR shedding by suppressing expression 
      of TACE. In addition, persicarin and I3G reduced PMA-stimulated phosphorylation 
      of p38MAPK, extracellular regulated kinases (ERK) 1/2, and c-Jun N-terminal 
      kinase (JNK). Given these results, persicarin and I3G could be used as a 
      candidate therapeutic for treatment of severe vascular inflammatory diseases.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Ku, Sae-Kwang
AU  - Ku SK
AD  - Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany 
      University, Gyeongsan, 712-715, Republic of Korea.
FAU - Han, Min-Su
AU  - Han MS
FAU - Bae, Jong-Sup
AU  - Bae JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130530
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Antigens, CD)
RN  - 0 (Antioxidants)
RN  - 0 (Endothelial Protein C Receptor)
RN  - 0 (Flavones)
RN  - 0 (Galactosides)
RN  - 0 (PROCR protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (isorhamnetin-3-O-galactoside)
RN  - 0 (persicarin)
RN  - 56937-68-9 (phorbolol myristate acetate)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
RN  - EC 3.4.24.86 (Adam17 protein, mouse)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - ADAM Proteins/genetics
MH  - ADAM17 Protein
MH  - Animals
MH  - Antigens, CD/genetics/*metabolism
MH  - Antioxidants/isolation & purification/*pharmacology
MH  - Down-Regulation/*drug effects
MH  - Endothelial Protein C Receptor
MH  - Enzyme Activation/drug effects
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Flavones/isolation & purification/*pharmacology
MH  - Galactosides/isolation & purification/*pharmacology
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - MAP Kinase Kinase 4/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oenanthe/chemistry
MH  - Quercetin/*analogs & derivatives/isolation & purification/pharmacology
MH  - Receptors, Cell Surface/genetics/*metabolism
MH  - Tetradecanoylphorbol Acetate/analogs & derivatives/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - APC
OT  - EPCR
OT  - ERK
OT  - I3G
OT  - JNK
OT  - PAR-1
OT  - PMA
OT  - TACE
OT  - activated protein C
OT  - c-Jun N-terminal kinase
OT  - endothelial protein C receptor
OT  - extracellular regulated kinase
OT  - isorhamnetin-3-O-galactoside
OT  - persicarin
OT  - phorbol-12-myristate 13-acetate
OT  - protease activated receptor-1
OT  - sEPCR
OT  - shedding
OT  - soluble EPCR
OT  - tumor necrosis factor-alpha converting enzyme
EDAT- 2013/06/04 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/06/04 06:00
PHST- 2013/01/22 00:00 [received]
PHST- 2013/05/04 00:00 [revised]
PHST- 2013/05/05 00:00 [accepted]
PHST- 2013/06/04 06:00 [entrez]
PHST- 2013/06/04 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - S0049-3848(13)00196-5 [pii]
AID - 10.1016/j.thromres.2013.05.004 [doi]
PST - ppublish
SO  - Thromb Res. 2013 Jul;132(1):e58-63. doi: 10.1016/j.thromres.2013.05.004. Epub 
      2013 May 30.