PMID- 23727504
OWN - NLM
STAT- MEDLINE
DCOM- 20140318
LR  - 20211021
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 247
DP  - 2013 Sep 5
TI  - Sexually dimorphic and developmentally regulated expression of tubulin-specific 
      chaperone protein A in the LMAN of zebra finches.
PG  - 182-90
LID - S0306-4522(13)00452-1 [pii]
LID - 10.1016/j.neuroscience.2013.05.032 [doi]
AB  - Sex differences in brain and behavior exist across vertebrates, but the molecular 
      factors regulating their development are largely unknown. Songbirds exhibit 
      substantial sexual dimorphisms. In zebra finches, only males sing, and the brain 
      areas regulating song learning and production are much larger in males. Recent 
      data suggest that sex chromosome genes (males ZZ; females ZW) may play roles in 
      sexual differentiation. The present studies tested the hypothesis that a Z-gene, 
      tubulin-specific chaperone protein A (TBCA), contributes to sexual 
      differentiation of the song system. This taxonomically conserved gene is integral 
      to microtubule synthesis, and within the song system, its mRNA is specifically 
      increased in males compared to females in the lateral magnocellular nucleus of 
      the anterior nidopallium (LMAN), a region critical for song learning and 
      plasticity. Using in situ hybridization, Western blot analysis, and 
      immunohistochemistry, we observed effects of both age and sex on TBCA mRNA and 
      protein expression. The transcript is increased in males compared to females at 
      three juvenile ages, but not in adults. TBCA protein, both the number of 
      immunoreactive cells and relative concentration in LMAN, is diminished in adults 
      compared to juveniles. The latter was also increased in males compared to females 
      at post-hatching day 25. With double-label immunofluorescence and retrograde 
      tract tracing, we also document that the majority of TBCA+ cells in LMAN are 
      neurons, and that they include robust nucleus of the arcopallium-projecting 
      cells. These results indicate that TBCA is both temporally and spatially primed 
      to facilitate the development of a sexually dimorphic neural pathway critical for 
      song.
CI  - Copyright (c) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Qi, L M
AU  - Qi LM
AD  - Neuroscience Program, Michigan State University, East Lansing, MI 48824-1101, 
      USA. qilinda@msu.edu
FAU - Wade, J
AU  - Wade J
LA  - eng
GR  - R01 MH055488/MH/NIMH NIH HHS/United States
GR  - R01 MH096705/MH/NIMH NIH HHS/United States
GR  - R01-MH096705/MH/NIMH NIH HHS/United States
GR  - R01-MH55488/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130529
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (TBCA protein, human)
SB  - IM
MH  - Animals
MH  - Brain/*physiology
MH  - Female
MH  - Finches
MH  - *Gene Expression Regulation, Developmental
MH  - Male
MH  - Microtubule-Associated Proteins/*biosynthesis/genetics/physiology
MH  - Molecular Chaperones/*biosynthesis/genetics/physiology
MH  - Neural Pathways/physiology
MH  - Random Allocation
MH  - *Sex Characteristics
MH  - Vocalization, Animal/physiology
PMC - PMC3754799
MID - NIHMS498820
OTO - NOTNLM
OT  - ANOVA
OT  - HRP
OT  - HVC
OT  - LMAN
OT  - NGS
OT  - PBS
OT  - RA
OT  - TBCA
OT  - TRITC
OT  - analysis of variance
OT  - high vocal center
OT  - horseradish peroxidase
OT  - lateral magnocellular nucleus of the anterior nidopallium
OT  - neural development
OT  - normal goat serum
OT  - phosphate-buffered saline
OT  - robust nucleus of the arcopallium
OT  - sex chromosome
OT  - sex difference
OT  - song system
OT  - tetramethyl rhodamine isothiocyanate
OT  - tubulin-specific chaperone protein A
EDAT- 2013/06/04 06:00
MHDA- 2014/03/19 06:00
PMCR- 2014/09/05
CRDT- 2013/06/04 06:00
PHST- 2013/04/12 00:00 [received]
PHST- 2013/05/16 00:00 [revised]
PHST- 2013/05/17 00:00 [accepted]
PHST- 2013/06/04 06:00 [entrez]
PHST- 2013/06/04 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
PHST- 2014/09/05 00:00 [pmc-release]
AID - S0306-4522(13)00452-1 [pii]
AID - 10.1016/j.neuroscience.2013.05.032 [doi]
PST - ppublish
SO  - Neuroscience. 2013 Sep 5;247:182-90. doi: 10.1016/j.neuroscience.2013.05.032. 
      Epub 2013 May 29.