PMID- 23727804
OWN - NLM
STAT- MEDLINE
DCOM- 20140321
LR  - 20190923
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 23
IP  - 8
DP  - 2013 Aug
TI  - In vitro screening for compounds derived from traditional chinese medicines with 
      antiviral activities against porcine reproductive and respiratory syndrome virus.
PG  - 1076-83
AB  - Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested 
      for their antiviral activity against porcine reproductive and respiratory 
      syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect 
      (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium 
      bromide test were used to determine the 50% cytotoxic concentration (CC50) and 
      50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested 
      compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. 
      The EC50 values were 270.8 +/- 14.6 mug/ml and 28.21 +/- 26.0 mug/ml and the 
      selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and 
      virucidal assay indicated that the anti-PRRSV activity of the two compounds could 
      be due to their inhibiting the early stage of virus replication and/or 
      inactivating the virus directly. The inhibition of the virus attachment was not 
      observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic 
      acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum 
      non-cytotoxic concentrations. The results have provided a basis for further 
      exploration of their antiviral properties and mechanisms in vivo. We believe that 
      the chlorogenic acid and scutellarin have a great potential to be developed as 
      new anti-PRRSV drugs for clinical application.
FAU - Cheng, Jia
AU  - Cheng J
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR of China.
FAU - Sun, Na
AU  - Sun N
FAU - Zhao, Xin
AU  - Zhao X
FAU - Niu, Li
AU  - Niu L
FAU - Song, Meiqin
AU  - Song M
FAU - Sun, Yaogui
AU  - Sun Y
FAU - Jiang, Junbing
AU  - Jiang J
FAU - Guo, Jianhua
AU  - Guo J
FAU - Bai, Yuansheng
AU  - Bai Y
FAU - He, Junping
AU  - He J
FAU - Li, Hongquan
AU  - Li H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (Antiviral Agents)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Antiviral Agents/isolation & purification/*pharmacology
MH  - China
MH  - Cytopathogenic Effect, Viral/drug effects
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Plants, Medicinal/*chemistry
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Virus Inactivation
MH  - Virus Replication/drug effects
EDAT- 2013/06/04 06:00
MHDA- 2014/03/22 06:00
CRDT- 2013/06/04 06:00
PHST- 2013/06/04 06:00 [entrez]
PHST- 2013/06/04 06:00 [pubmed]
PHST- 2014/03/22 06:00 [medline]
AID - 10.4014/jmb.1303.03074 [pii]
AID - 10.4014/jmb.1303.03074 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2013 Aug;23(8):1076-83. doi: 10.4014/jmb.1303.03074.