PMID- 23727805
OWN - NLM
STAT- MEDLINE
DCOM- 20140321
LR  - 20190923
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 23
IP  - 8
DP  - 2013 Aug
TI  - Orientia tsutsugamushi infection induces CD4+ T cell activation via human 
      dendritic cell activity.
PG  - 1159-66
AB  - Orientia tsutsugamushi, a gram-negative bacterium, causes severe acute febrile 
      illness in humans. Despite this danger, the route of infection, infectivity, and 
      protective mechanisms of the host's immune response to O. tsutsugamushi are 
      unclear. Dendritic cells (DCs) are one of the most important cell types in 
      bridging the innate and adaptive immune responses. In this study, we observed 
      that O. tsutsugamushi infects and replicates in monocyte-derived DCs (MODCs). 
      During infection and replication, the expressions of the cytokines IL-12 and 
      TNF-alpha, as well as the co-stimulatory molecules CD80, CD83, CD86, and CD40, were 
      increased in MODCs. When O. tsutsugamushi-treated MODCs were co-cultured with 
      autologous CD4(+) T cells, they enhanced production of IFN-gamma, a major Th1 
      cytokine. Collectively, our results show that O. tsutsugamushi can replicate in 
      MODCs and can simultaneously induce MODC maturation and increase proinflammatory 
      cytokine levels in MODCs that subsequently activate CD4(+) T cells.
FAU - Chu, Hyuk
AU  - Chu H
AD  - Animal Science and Biotechnology Major and WCU Biomodulation Major, Department of 
      Agricultural Biotechnology, and Research Institute for Agriculture and Life 
      Sciences, and Center for Food and Bioconvergence, Seoul National University, 
      Seoul 151-921, Republic of Korea.
FAU - Park, Sung-Moo
AU  - Park SM
FAU - Cheon, In Su
AU  - Cheon IS
FAU - Park, Mi-Yeoun
AU  - Park MY
FAU - Shim, Byoung-Shik
AU  - Shim BS
FAU - Gil, Byoung-Cheol
AU  - Gil BC
FAU - Jeung, Woon Hee
AU  - Jeung WH
FAU - Hwang, Kyu-Jam
AU  - Hwang KJ
FAU - Song, Ki-Duk
AU  - Song KD
FAU - Hong, Kee-Jong
AU  - Hong KJ
FAU - Song, Manki
AU  - Song M
FAU - Jeong, Hang-Jin
AU  - Jeong HJ
FAU - Han, Seung Hyun
AU  - Han SH
FAU - Yun, Cheol-Heui
AU  - Yun CH
LA  - eng
GR  - 2010-N52001-00/PHS HHS/United States
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
SB  - IM
MH  - Antigens, CD/biosynthesis/genetics
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/biosynthesis/genetics
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Gene Expression Profiling
MH  - Humans
MH  - Orientia tsutsugamushi/*immunology
MH  - Scrub Typhus/*immunology/*microbiology
EDAT- 2013/06/04 06:00
MHDA- 2014/03/22 06:00
CRDT- 2013/06/04 06:00
PHST- 2013/06/04 06:00 [entrez]
PHST- 2013/06/04 06:00 [pubmed]
PHST- 2014/03/22 06:00 [medline]
AID - 10.4014/jmb.1303.03019 [pii]
AID - 10.4014/jmb.1303.03019 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2013 Aug;23(8):1159-66. doi: 10.4014/jmb.1303.03019.