PMID- 23727982
OWN - NLM
STAT- MEDLINE
DCOM- 20140221
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 36
IP  - 8
DP  - 2013
TI  - Stem cell factor combined with matrix proteins regulates the attachment and 
      migration of melanocyte precursors of human hair follicles in vitro.
PG  - 1317-25
AB  - In conjunction with matrix proteins, stem cell factor (SCF) plays an important 
      role in the migration of melanocyte precursors (MPs) derived from the mouse 
      embryo. However, no studies have demonstrated an effect of SCF on human 
      follicular MPs migration in vitro. In this report, first we demonstrate the 
      immature state of the follicular MPs. Then cell attachment rate was measured by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Standard 
      48-well chemotaxis chambers were used for a transfilter migration assay. F-actin 
      was labeled by rhodamine-conjugated phalloidin, and then organization of the 
      actin cytoskeleton was observed by confocal microscope. In the results, we 
      directly show that MPs adhere more strongly to fibronectin (FN), laminin (LN) and 
      type IV collagen (CIV) than to the negative control. SCF decreased the adhesion 
      of MPs to FN and CIV. A chemotaxis analysis showed that FN and CIV have 
      chemotactic effects on MPs. FN showed an obvious increase in chemotactic effects 
      on MPs with SCF treatment comparing with the control group, but there were no 
      significant changes in the levels of chemotaxis with CIV and LN when the cells 
      were treated with SCF. SCF was chemotactic to MPs, and the presence of FN caused 
      a statistically significant increase in MPs migration at various concentrations 
      of SCF. Furthermore, we showed that SCF, in combination with FN, could induce an 
      apparent increase in actin stress fiber formation in MPs. Our results indicate 
      that SCF, in combination with matrix proteins and in particular with FN, 
      regulates the movement of MPs by both altering cell attachment and increasing 
      cell chemotaxis.
FAU - Wang, Da-guang
AU  - Wang DG
AD  - Department of Dermatology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing 210029, P. R. China.
FAU - Xu, Xi-hui
AU  - Xu XH
FAU - Ma, Hui-jun
AU  - Ma HJ
FAU - Li, Cheng-rang
AU  - Li CR
FAU - Yue, Xue-zhuang
AU  - Yue XZ
FAU - Gao, Jie
AU  - Gao J
FAU - Zhu, Wen-yuan
AU  - Zhu WY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130603
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Collagen Type IV)
RN  - 0 (Fibronectins)
RN  - 0 (Laminin)
RN  - 0 (Stem Cell Factor)
SB  - IM
MH  - Adult
MH  - Cell Adhesion/physiology
MH  - Cell Movement/physiology
MH  - Cells, Cultured
MH  - Collagen Type IV/*metabolism
MH  - Fibronectins/*metabolism
MH  - Hair Follicle/*cytology
MH  - Humans
MH  - Laminin/*metabolism
MH  - Male
MH  - Melanocytes/*cytology
MH  - Middle Aged
MH  - Stem Cell Factor/metabolism/*pharmacology
EDAT- 2013/06/04 06:00
MHDA- 2014/02/22 06:00
CRDT- 2013/06/04 06:00
PHST- 2013/06/04 06:00 [entrez]
PHST- 2013/06/04 06:00 [pubmed]
PHST- 2014/02/22 06:00 [medline]
AID - DN/JST.JSTAGE/bpb/b13-00172 [pii]
AID - 10.1248/bpb.b13-00172 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2013;36(8):1317-25. doi: 10.1248/bpb.b13-00172. Epub 2013 Jun 3.