PMID- 23728104
OWN - NLM
STAT- MEDLINE
DCOM- 20140109
LR  - 20201209
IS  - 1108-7161 (Print)
IS  - 1108-7161 (Linking)
VI  - 13
IP  - 2
DP  - 2013 Jun
TI  - Time-dependent effects of sclerostin antibody on a mouse fracture healing model.
PG  - 178-84
AB  - OBJECTIVES: Treatment with Sclerostin antibody (Scl-Ab) has shown to enhance 
      fracture healing in rodent and non-human primate models. The current study 
      investigated the time-dependent changes during Scl-Ab treatment in a mouse 
      osteotomy model. METHODS: 1 day after osteotomy, C57BL mice received subcutaneous 
      injection with vehicle or Scl-Ab at 25 mg/kg, twice/week for 2, 4, or 6 weeks. 20 
      mice from each group were necropsied at weeks 2, 4, and 6 for Micro-CT, 
      histomorphmetry and mechanical testing examinations. RESULTS: The bone mineral 
      apposition rate at fracture callus was significantly higher in the Scl-Ab treated 
      groups at all the time points. Micro-CT analysis showed that the volumetric bone 
      mineral density (vBMD) and bone volume over tissue volume (BV/TV) in the Scl-Ab 
      treated groups at 4 and 6 weeks were significantly greater than that of vehicle 
      control groups. Mechanical testing showed that the maximum load of failure at the 
      fracture callus increased significantly by 68% at 6 weeks in the Scl-Ab treated 
      groups. CONCLUSIONS: This study confirmed that mice treated with Scl-Ab increased 
      bone formation from 2 weeks, bone mineral density and bone volume at 4 weeks, 
      followed by significant increase in bone strength at the fracture site at 6 
      weeks. These results suggest that applying sclerostin antibody at early stage 
      fracture healing promotes fracture healing.
FAU - Cui, L
AU  - Cui L
AD  - Guangdong Key Laboratory for R&D of Natural Drug, Guangdong Medical College, 
      Zhanjiang 524023, Guangdong, PR China.
FAU - Cheng, H
AU  - Cheng H
FAU - Song, C
AU  - Song C
FAU - Li, C
AU  - Li C
FAU - Simonet, W S
AU  - Simonet WS
FAU - Ke, H Z
AU  - Ke HZ
FAU - Li, G
AU  - Li G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - J Musculoskelet Neuronal Interact
JT  - Journal of musculoskeletal & neuronal interactions
JID - 101084496
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antibodies)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Sost protein, mouse)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Antibodies/*pharmacology
MH  - Biomechanical Phenomena
MH  - Bone Density/drug effects
MH  - Femoral Fractures/diagnostic imaging/drug therapy
MH  - Fracture Healing/*drug effects
MH  - Fractures, Bone/diagnostic imaging/*drug therapy
MH  - Glycoproteins/*immunology
MH  - Image Processing, Computer-Assisted
MH  - Intercellular Signaling Peptides and Proteins
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Fluorescence
MH  - Osteotomy
MH  - Tissue Fixation
MH  - Tomography, X-Ray Computed
EDAT- 2013/06/04 06:00
MHDA- 2014/01/10 06:00
CRDT- 2013/06/04 06:00
PHST- 2013/06/04 06:00 [entrez]
PHST- 2013/06/04 06:00 [pubmed]
PHST- 2014/01/10 06:00 [medline]
PST - ppublish
SO  - J Musculoskelet Neuronal Interact. 2013 Jun;13(2):178-84.