PMID- 23729169
OWN - NLM
STAT- MEDLINE
DCOM- 20140131
LR  - 20211021
IS  - 1432-0886 (Electronic)
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 122
IP  - 4
DP  - 2013 Aug
TI  - Alteration of poly(ADP-ribose) metabolism affects murine sperm nuclear 
      architecture by impairing pericentric heterochromatin condensation.
PG  - 319-35
LID - 10.1007/s00412-013-0416-y [doi]
AB  - The mammalian sperm nucleus is characterized by unique properties that are 
      important for fertilization. Sperm DNA retains only small numbers of histones in 
      distinct positions, and the majority of the genome is protamine associated, which 
      allows for extreme condensation and protection of the genetic material. 
      Furthermore, sperm nuclei display a highly ordered architecture that is 
      characterized by a centrally located chromocenter comprising the pericentromeric 
      chromosome regions and peripherally positioned telomeres. Establishment of this 
      unique and well-conserved nuclear organization during spermiogenesis is not well 
      understood. Utilizing fluorescence in situ hybridization (FISH), we show that a 
      large fraction of the histone-associated sperm genome is repetitive in nature, 
      while a smaller fraction is associated with unique DNA sequences. Coordinated 
      activity of poly(ADP-ribose) (PAR) polymerase and topoisomerase II beta has been 
      shown to facilitate DNA relaxation and histone to protamine transition during 
      spermatid condensation, and altered PAR metabolism is associated with an increase 
      in sperm histone content. Combining FISH with three-dimensional laser scanning 
      microscopy technology, we further show that altered PAR metabolism by genetic or 
      pharmacological intervention leads to a disturbance of the overall sperm nuclear 
      architecture with a lower degree of organization and condensation of the 
      chromocenters formed by chromosomal pericentromeric heterochromatin.
FAU - Meyer-Ficca, Mirella L
AU  - Meyer-Ficca ML
AD  - Center for Animal Transgenesis and Germ Cell Research, Department of Animal 
      Biology and Mari Lowe Center for Comparative Oncology, University of Pennsylvania 
      School of Veterinary Medicine, Philadelphia, PA 19104, USA.
FAU - Lonchar, Julia D
AU  - Lonchar JD
FAU - Ihara, Motomasa
AU  - Ihara M
FAU - Bader, Jessica J
AU  - Bader JJ
FAU - Meyer, Ralph G
AU  - Meyer RG
LA  - eng
GR  - U54 HD068157/HD/NICHD NIH HHS/United States
GR  - R01 HD048837/HD/NICHD NIH HHS/United States
GR  - U54HD068157/HD/NICHD NIH HHS/United States
GR  - P50 HD068157/HD/NICHD NIH HHS/United States
GR  - R01HD048837/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130601
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Heterochromatin)
RN  - 0 (Histones)
RN  - 26656-46-2 (Poly Adenosine Diphosphate Ribose)
RN  - 9007-49-2 (DNA)
RN  - EC 2.4.2.30 (Parp1 protein, mouse)
RN  - EC 2.4.2.30 (Poly (ADP-Ribose) Polymerase-1)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/genetics/*metabolism
MH  - *Chromatin Assembly and Disassembly
MH  - DNA/genetics/metabolism
MH  - Heterochromatin/*metabolism
MH  - Histones/metabolism
MH  - Male
MH  - Mice/genetics/*metabolism
MH  - Mice, Knockout
MH  - Poly (ADP-Ribose) Polymerase-1
MH  - Poly Adenosine Diphosphate Ribose/*metabolism
MH  - Poly(ADP-ribose) Polymerases/genetics/metabolism
MH  - Spermatogenesis
MH  - Spermatozoa/cytology/*metabolism
PMC - PMC3762462
MID - NIHMS487717
EDAT- 2013/06/05 06:00
MHDA- 2014/02/01 06:00
PMCR- 2014/08/01
CRDT- 2013/06/05 06:00
PHST- 2013/03/12 00:00 [received]
PHST- 2013/05/13 00:00 [accepted]
PHST- 2013/05/07 00:00 [revised]
PHST- 2013/06/05 06:00 [entrez]
PHST- 2013/06/05 06:00 [pubmed]
PHST- 2014/02/01 06:00 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.1007/s00412-013-0416-y [doi]
PST - ppublish
SO  - Chromosoma. 2013 Aug;122(4):319-35. doi: 10.1007/s00412-013-0416-y. Epub 2013 Jun 
      1.