PMID- 23729636
OWN - NLM
STAT- MEDLINE
DCOM- 20140206
LR  - 20191210
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 37
IP  - 6
DP  - 2013 Jul-Aug
TI  - Determination of MDMA and its three metabolites in the rat perfused liver.
PG  - 357-61
LID - 10.1093/jat/bkt039 [doi]
AB  - 3,4-Methylenedioxymethamphetamine (MDMA) is one of the most commonly abused 
      illicit drugs in the world. We developed a rapid and simple high-performance 
      liquid chromatography with a fluorescence (FL) detector method to determine MDMA 
      and its metabolites, such as 3,4-methylenedioxyamphetamine (MDA), 
      4-hydroxy-3-methoxyamphetamine (HMA) and its main unstable metabolite 
      3,4-dihydroxymethamphetamine (HHMA) besides the internal standards, in a 
      perfusion medium. The separation of analytes was performed at 25 degrees C on a 
      Chromolith(R) C18 (100 x 4.6 mm) column from Merck (Darmstadt, Germany) without any 
      derivatization. The FL detector wavelength was fixed at 285 nm for excitation and 
      at 320 nm for emission. Acetonitrile:phosphate buffer (0.02 M) at pH = 3 (5:95 
      v/v) was used as the mobile phase. The elution order was HHMA, HMA, MDA and MDMA 
      with a retention time of 1.7, 2.6, 6.1 and 7.4 min, respectively. The method was 
      validated according to the FDA bioanalytical method validation guideline. The 
      limits of quantifications (LOQs) obtained for MDMA, MDA, HMA and HHMA were 1, 1, 
      1.5 and 5 ng/mL, respectively. The repeatability of relative standard deviation 
      was  <11% (except for LOQs). This method was applied successfully to determine 
      MDMA and its metabolites in rat liver perfusion samples. To our knowledge, this 
      is the first method introduced for the determination of HHMA as a free form with 
      an FL detector.
FAU - Jamali, Bardia
AU  - Jamali B
AD  - Biopharmaceutics and Pharmacokinetics Division, Department of Pharmaceutics, 
      Faculty of Pharmacy, Tehran University of Medical Sciences, Iran.
FAU - Ardakani, Yalda Hosseinzadeh
AU  - Ardakani YH
FAU - Foroumadi, Alireza
AU  - Foroumadi A
FAU - Kobarfard, Farzad
AU  - Kobarfard F
FAU - Rouini, Mohammad-Reza
AU  - Rouini MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130531
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Illicit Drugs)
RN  - 13026-44-3 (3-O-methyl-alpha-methyldopamine)
RN  - 15398-87-5 (alpha-methylepinine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - R7339QLN1C (Deoxyepinephrine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/metabolism
MH  - Animals
MH  - Chromatography, High Pressure Liquid/methods
MH  - Deoxyepinephrine/analogs & derivatives/metabolism
MH  - Dopamine/analogs & derivatives/metabolism
MH  - Illicit Drugs/adverse effects/*analysis
MH  - Limit of Detection
MH  - Liver/*drug effects/metabolism
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Substance Abuse Detection/methods
EDAT- 2013/06/05 06:00
MHDA- 2014/02/07 06:00
CRDT- 2013/06/05 06:00
PHST- 2013/06/05 06:00 [entrez]
PHST- 2013/06/05 06:00 [pubmed]
PHST- 2014/02/07 06:00 [medline]
AID - bkt039 [pii]
AID - 10.1093/jat/bkt039 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2013 Jul-Aug;37(6):357-61. doi: 10.1093/jat/bkt039. Epub 2013 May 
      31.