PMID- 23731191
OWN - NLM
STAT- MEDLINE
DCOM- 20140106
LR  - 20191210
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 30
IP  - 5
DP  - 2013
TI  - A simple method for the determination of fluoroquinolone residues in tilapia 
      (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS 
      QToF.
PG  - 813-25
LID - 10.1080/19440049.2013.791750 [doi]
AB  - The use of antimicrobials in livestock production is a powerful resource applied 
      throughout the world to guarantee high yield and control bacterial diseases in 
      aquaculture. However, residues of these substances in animal products represent a 
      potential risk to consumer health when residue levels are above the established 
      maximum residue limits (MRLs). Fluoroquinolones (FQs) are antimicrobials commonly 
      used worldwide in aquaculture. The aim of this work was to develop and validate a 
      simple analytical method for the simultaneous determination of norfloxacin, 
      danofloxacin, enrofloxacin and ciprofloxacin levels in tilapia (Oreochromis 
      niloticus) and pacu (Piaractus mesopotamicus) fillets using liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS) quadrupole time of flight 
      (QToF). The FQs were extracted from the fillets with 1% acetic acid-methanol and 
      1% acetic acid-acetonitrile solutions using ultrasonic assistance. The clean-up 
      was performed with hexane. Chromatographic separation was conducted in an XTerra 
      RP18 column (2.1 x 150 mm, 5 microm) at 25  degrees C with a flow of 0.2 mL min(-)(1). The mobile 
      phase consisted of 0.1% aqueous formic acid and acetonitrile, with gradient 
      elution. The validation parameters for all FQs were linearity (>0.99), intra-day 
      precision (CV of 1%-9%), inter-day precision (CV of 3%-17%), decision limit 
      (63-126 ng g(-)(1)), detection capability (76 -152 ng g(-)(1)) and accuracy (90%-111%). 
      The limit of quantification was lower than the MRL for each FQ, indicating that 
      the method is suitable for the determination of the FQ levels in the fish 
      fillets. The mass analyser of the QToF type was able to confirm the identities of 
      the FQs with an error of the accuracy of the mass (reasons m/z) of less than 10 
      ppm.
FAU - Quesada, Silvia Pilco
AU  - Quesada SP
AD  - Department of Food Science , School of Food Engineering , University of Campinas, 
      Rua Monteiro Lobato 80, 13083-862 Campinas, SP, Brazil.
FAU - Paschoal, Jonas Augusto Rizzato
AU  - Paschoal JA
FAU - Reyes, Felix Guillermo
AU  - Reyes FG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20130603
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fluoroquinolones)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - *Characidae
MH  - Chromatography, Liquid/*methods
MH  - Drug Residues/*analysis
MH  - Fluoroquinolones/*analysis
MH  - Reference Standards
MH  - Tandem Mass Spectrometry/*methods
MH  - *Tilapia
EDAT- 2013/06/05 06:00
MHDA- 2014/01/07 06:00
CRDT- 2013/06/05 06:00
PHST- 2013/06/05 06:00 [entrez]
PHST- 2013/06/05 06:00 [pubmed]
PHST- 2014/01/07 06:00 [medline]
AID - 10.1080/19440049.2013.791750 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(5):813-25. 
      doi: 10.1080/19440049.2013.791750. Epub 2013 Jun 3.