PMID- 23731762
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20220408
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 15
IP  - 7
DP  - 2013 Jul
TI  - Combined treatment with platelet-rich plasma and brain-derived neurotrophic 
      factor-overexpressing bone marrow stromal cells supports axonal remyelination in 
      a rat spinal cord hemi-section model.
PG  - 792-804
LID - S1465-3249(13)00500-8 [pii]
LID - 10.1016/j.jcyt.2013.04.004 [doi]
AB  - BACKGROUND AIMS: Combining biologic matrices is becoming a better choice to 
      advance stem cell-based therapies. Platelet-rich plasma (PRP) is a biologic 
      product of concentrated platelets and has been used to promote regeneration of 
      peripheral nerves after injury. We examined whether PRP could induce rat bone 
      marrow stromal cells (BMSCs) differentiation in vitro and whether a combination 
      of BMSCs, PRP and brain-derived neurotrophic factor (BDNF) could provide additive 
      therapeutic benefits in vivo after spinal cord injury (SCI). METHODS: BMSCs and 
      BDNF-secreting BMSCs (BDNF-BMSCs) were cultured with PRP for 7 days and 21 days, 
      respectively, and neurofilament (NF)-200, glial fibrillary acidic protein (GFAP), 
      microtubule-associated protein 2 (MAP2) and ribosomal protein S6 kinase (p70S6K) 
      gene levels were assessed. After T10 hemi-section in 102 rats, 15-muL scaffolds 
      (PRP alone, BMSCs, PRP/BMSCs, BDNF-BMSCs or PRP/BDNF-BMSCs) were transplanted 
      into the lesion area, and real-time polymerase chain reaction, Western blot, 
      immunohistochemistry and ultrastructural studies were performed. RESULTS: The 
      messenger RNA expression of NF-200, GFAP, MAP2 and p70S6K was promoted in BMSCs 
      and BDNF-BMSCs after culture with PRP in vitro. BDNF levels were significantly 
      higher in the injured spinal cord after implantation of BDNF-BMSCs. In the 
      PRP/BDNF-BMSCs group at 8 weeks postoperatively, more GFAP was observed, with 
      less accumulation of astrocytes at the graft-host interface. Rats that received 
      PRP and BDNF-BMSC implants showed enhanced hind limb locomotor performance at 8 
      weeks postoperatively compared with control animals, with more axonal 
      remyelination. CONCLUSIONS: A combined treatment comprising PRP and 
      BDNF-overexpressing BMSCs produced beneficial effects in rats with regard to 
      functional recovery after SCI through enhancing migration of astrocytes into the 
      transplants and axonal remyelination.
CI  - Copyright (c) 2013 International Society for Cellular Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Zhao, Tengfei
AU  - Zhao T
AD  - The Second Affiliated Hospital (Binjiang Branch) Zhejiang University School of 
      Medicine, Hangzhou Binjiang Hospital, Hangzhou, China.
FAU - Yan, Weiqi
AU  - Yan W
FAU - Xu, Kan
AU  - Xu K
FAU - Qi, Yiying
AU  - Qi Y
FAU - Dai, Xuesong
AU  - Dai X
FAU - Shi, Zhongli
AU  - Shi Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Animals
MH  - Axons/metabolism/*transplantation
MH  - Bone Marrow Transplantation
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells
MH  - *Nerve Regeneration
MH  - Platelet-Rich Plasma/chemistry
MH  - Rats
MH  - Recovery of Function
MH  - Spinal Cord Injuries/pathology/physiopathology/*therapy
EDAT- 2013/06/05 06:00
MHDA- 2014/01/01 06:00
CRDT- 2013/06/05 06:00
PHST- 2012/07/19 00:00 [received]
PHST- 2013/03/24 00:00 [revised]
PHST- 2013/04/07 00:00 [accepted]
PHST- 2013/06/05 06:00 [entrez]
PHST- 2013/06/05 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - S1465-3249(13)00500-8 [pii]
AID - 10.1016/j.jcyt.2013.04.004 [doi]
PST - ppublish
SO  - Cytotherapy. 2013 Jul;15(7):792-804. doi: 10.1016/j.jcyt.2013.04.004.