PMID- 23731954
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20130812
IS  - 1558-1497 (Electronic)
IS  - 0197-4580 (Linking)
VI  - 34
IP  - 11
DP  - 2013 Nov
TI  - Improvement of cognitive function and physical activity of aging mice by human 
      neural stem cells over-expressing choline acetyltransferase.
PG  - 2639-46
LID - S0197-4580(13)00194-2 [pii]
LID - 10.1016/j.neurobiolaging.2013.04.026 [doi]
AB  - Aging is characterized by progressive loss of cognitive and memory functions as 
      well as decrease in physical activities. In the present study, a human neural 
      stem cell line (F3 NSC) over-expressing choline acetyltransferase (F3.ChAT), an 
      enzyme responsible for acetylcholine synthesis, was generated and transplanted in 
      the brain of 18-month-old male ICR mice. Four weeks post-transplantation, 
      neurobehavioral functions, expression of ChAT enzyme, production of acetylcholine 
      and neurotrophic factors, and expression of cholinergic nervous system markers in 
      transplanted animals were investigated. F3.ChAT NSCs markedly improved both the 
      cognitive function and physical activity of aging animals, in parallel with the 
      elevation of brain acetylcholine level. Transplanted F3 and F3.ChAT cells were 
      found to differentiate into neurons and astrocytes, and to produce ChAT proteins. 
      Transplantation of the stem cells increased brain-derived neurotrophic factor 
      (BDNF) and nerve growth factor (NGF), enhanced expression of Trk B, and restored 
      host microtubule-associated protein 2 and cholinergic nervous system. The results 
      demonstrate that human NSCs over-expressing ChAT improve cognitive function and 
      physical activity of aging mice, not only by producing ACh directly but also by 
      restoring cholinergic neuronal integrity, which might be mediated by 
      neurotrophins BDNF and NGF.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Park, Dongsun
AU  - Park D
AD  - College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic 
      of Korea.
FAU - Yang, Yun-Hui
AU  - Yang YH
FAU - Bae, Dae Kwon
AU  - Bae DK
FAU - Lee, Sun Hee
AU  - Lee SH
FAU - Yang, Goeun
AU  - Yang G
FAU - Kyung, Jangbeen
AU  - Kyung J
FAU - Kim, Dajeong
AU  - Kim D
FAU - Choi, Ehn-Kyoung
AU  - Choi EK
FAU - Lee, Seong Won
AU  - Lee SW
FAU - Kim, Gon Hyung
AU  - Kim GH
FAU - Hong, Jin Tae
AU  - Hong JT
FAU - Choi, Kyung-Chul
AU  - Choi KC
FAU - Lee, Hong Jun
AU  - Lee HJ
FAU - Kim, Seung U
AU  - Kim SU
FAU - Kim, Yun-Bae
AU  - Kim YB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130531
PL  - United States
TA  - Neurobiol Aging
JT  - Neurobiology of aging
JID - 8100437
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Receptors, Cholinergic)
RN  - 108688-71-7 (neurofilament protein H)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/metabolism
MH  - Aging/pathology/*physiology
MH  - Animals
MH  - Brain/enzymology/metabolism
MH  - Choline O-Acetyltransferase/genetics/*metabolism
MH  - Cognition Disorders/etiology/*surgery
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Humans
MH  - Male
MH  - Maze Learning
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Motor Activity/*physiology
MH  - Neural Stem Cells/*physiology/transplantation
MH  - Neurofilament Proteins/metabolism
MH  - Receptors, Cholinergic/genetics/metabolism
MH  - Time Factors
MH  - Transfection
OTO - NOTNLM
OT  - Acetylcholine
OT  - Aging
OT  - Brain-derived neurotrophic factor
OT  - Choline acetyltransferase
OT  - Cognitive function
OT  - Human neural stem cell
OT  - Nerve growth factor
OT  - Physical activity
EDAT- 2013/06/05 06:00
MHDA- 2014/03/07 06:00
CRDT- 2013/06/05 06:00
PHST- 2012/10/03 00:00 [received]
PHST- 2013/04/18 00:00 [revised]
PHST- 2013/04/28 00:00 [accepted]
PHST- 2013/06/05 06:00 [entrez]
PHST- 2013/06/05 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
AID - S0197-4580(13)00194-2 [pii]
AID - 10.1016/j.neurobiolaging.2013.04.026 [doi]
PST - ppublish
SO  - Neurobiol Aging. 2013 Nov;34(11):2639-46. doi: 
      10.1016/j.neurobiolaging.2013.04.026. Epub 2013 May 31.