PMID- 2373521
OWN - NLM
STAT- MEDLINE
DCOM- 19900828
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 70
IP  - 2
DP  - 1990 Jun
TI  - The effect of platelet-activating factor on IgE binding to, and IgE-dependent 
      biological properties of, human eosinophils.
PG  - 251-7
AB  - This study investigated the effect of platelet-activating factor (PAF), 
      leukotriene B4 (LTB4) histamine and formyl-methionyl-leucyl-phenylalanine (FMLP) 
      on immunoglobulin E (IgE) binding and IgE-dependent cytotoxicity of human normal 
      density eosinophils. The binding of a native myeloma IgE to normal human 
      eosinophils was measured by flow cytometry using a fluorescein-conjugated 
      polyclonal anti-IgE antibody. Preincubation with PAF (optimal at 10(-7)M), but 
      not lyso-PAF or FMLP, gave dose-dependent increases in IgE binding. PAF and LTB4 
      gave significant increases in IgE binding after 5 min preincubation (P less than 
      0.05); the effect was further enhanced at 30 min (P less than 0.01). This was 
      further confirmed using the rosette assay where PAF and LTB4, but not lyso-PAF or 
      FMLP, gave dose- and time-dependent increases in IgE eosinophil rosettes. 
      Eosinophil cytotoxicity for schistosomula of Schistosoma mansoni, incubated with 
      immune serum, was also significantly enhanced (P less than 0.01) by PAF in a 
      dose-dependent fashion (optimal at 10(-8) M). Schistosomula coated with 
      FPLC-purified IgE fractions were susceptible to killing by normal density 
      eosinophils, and this was enhanced with PAF (10(-8)M), LTB4 (10(-7)M) and 
      histamine (10(-5)M) but not with FMLP (10(-7)M) or lyso-PAF. IgE-dependent 
      cytotoxicity was confirmed by the removal of contaminating IgG from IgE-rich 
      fractions, and by the abolishment of IgE-dependent cytotoxicity after IgE 
      adsorption. These results suggest that PAF (and to a lesser extent LTB4 and 
      histamine) increase IgE binding, IgE-dependent adherence and cytotoxicity of 
      normal human eosinophils. Although IgE receptors have not been identified, the 
      data support current concepts that certain biological properties of eosinophils 
      may be IgE associated.
FAU - Moqbel, R
AU  - Moqbel R
AD  - Department of Allergy and Clinical Immunology, National Heart & Lung Institute, 
      Brompton Hospital, London, U.K.
FAU - Walsh, G M
AU  - Walsh GM
FAU - Nagakura, T
AU  - Nagakura T
FAU - MacDonald, A J
AU  - MacDonald AJ
FAU - Wardlaw, A J
AU  - Wardlaw AJ
FAU - Iikura, Y
AU  - Iikura Y
FAU - Kay, A B
AU  - Kay AB
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Chemotactic Factors)
RN  - 0 (Platelet Activating Factor)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Cells, Cultured
MH  - Chemotactic Factors/pharmacology
MH  - Cytotoxicity, Immunologic/immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Eosinophils/*immunology/metabolism
MH  - Humans
MH  - Immunoglobulin E/immunology/*metabolism
MH  - Platelet Activating Factor/*pharmacology
MH  - Rosette Formation
PMC - PMC1384202
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
PMCR- 1991/06/01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PHST- 1991/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1990 Jun;70(2):251-7.