PMID- 23735731
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20131007
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 197
IP  - 3
DP  - 2013 Sep
TI  - Assessment of bone marrow infiltration diagnosed by flow cytometry in canine 
      large B cell lymphoma: prognostic significance and proposal of a cut-off value.
PG  - 776-81
LID - S1090-0233(13)00215-3 [pii]
LID - 10.1016/j.tvjl.2013.05.003 [doi]
AB  - The aims of this study were to assess the prognostic significance of bone marrow 
      (BM) infiltration in canine large B cell lymphoma (LBCL) and to establish cut-off 
      values for designating the BM as infiltrated by lymphoid blasts. The degree of BM 
      infiltration by large CD21 positive cells in dogs with LBCL was assessed by flow 
      cytometry (FC) and related to time to progression (TTP) and lymphoma-specific 
      survival (LSS). Forty-six dogs were prospectively enrolled, staged and treated 
      with a dose-intense chemotherapeutic protocol. BM infiltration was directly 
      correlated with peripheral blood infiltration (P=0.001), high lactate 
      dehydrogenase activity (P=0.0024) and substage b disease (P<0.001). In the 
      univariate analysis, there was a significant association between BM infiltration 
      diagnosed by FC and both TTP (P=0.001) and LSS (P<0.001). Substage was the only 
      factor associated with TTP in the multivariate analysis (P=0.002), whereas 
      substage (P<0.001) and anaemia (P=0.008) were associated with LSS. A cut-off of 
      3% BM infiltration had the strongest prognostic value, since it discriminated 
      between dogs with a poorer prognosis (median TTP 69 days; median LSS 155 days) 
      and dogs with a better prognosis (median TTP 149 days; median LSS 322 days). BM 
      analysis is an essential step in the staging of LBCL. The presence of BM 
      infiltration by FC at diagnosis is a negative prognostic indicator in canine 
      LBCL.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Marconato, Laura
AU  - Marconato L
AD  - Centro Oncologico Veterinario, via San Lorenzo 1-4, 40037 Sasso Marconi, BO, 
      Italy. Electronic address: lauramarconato@yahoo.it.
FAU - Martini, Valeria
AU  - Martini V
FAU - Aresu, Luca
AU  - Aresu L
FAU - Sampaolo, Michele
AU  - Sampaolo M
FAU - Valentini, Fabio
AU  - Valentini F
FAU - Rinaldi, Valentina
AU  - Rinaldi V
FAU - Comazzi, Stefano
AU  - Comazzi S
LA  - eng
PT  - Journal Article
DEP - 20130602
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
SB  - IM
MH  - Animals
MH  - Bone Marrow Diseases/pathology/*veterinary
MH  - Dog Diseases/diagnosis/*pathology
MH  - Dogs
MH  - Female
MH  - Flow Cytometry/*veterinary
MH  - Lymphoma, B-Cell/pathology/*veterinary
MH  - Male
MH  - Prognosis
OTO - NOTNLM
OT  - Bone marrow
OT  - Canine
OT  - Flow cytometry
OT  - Large B cell lymphoma
OT  - Prognosis
EDAT- 2013/06/06 06:00
MHDA- 2014/06/03 06:00
CRDT- 2013/06/06 06:00
PHST- 2012/12/03 00:00 [received]
PHST- 2013/04/29 00:00 [revised]
PHST- 2013/05/02 00:00 [accepted]
PHST- 2013/06/06 06:00 [entrez]
PHST- 2013/06/06 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
AID - S1090-0233(13)00215-3 [pii]
AID - 10.1016/j.tvjl.2013.05.003 [doi]
PST - ppublish
SO  - Vet J. 2013 Sep;197(3):776-81. doi: 10.1016/j.tvjl.2013.05.003. Epub 2013 Jun 2.