PMID- 23737133
OWN - NLM
STAT- MEDLINE
DCOM- 20140212
LR  - 20211021
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 381
IP  - 1-2
DP  - 2013 Sep
TI  - Vacuolar protein sorting 4B, an ATPase protein positively regulates the 
      progression of NSCLC via promoting cell division.
PG  - 163-71
LID - 10.1007/s11010-013-1699-2 [doi]
AB  - Vacuolar protein sorting 4B (VPS4B), a member of ATPase family proteins, plays a 
      crucial role in lysosome-dependent degradation. Recently, it was found that VPS4B 
      could negatively regulate breast cancer progression through promoting 
      lysosomal-dependent degradation for EGFR. Nevertheless, other studies found that 
      VPS4B was also essential for cell division and mitosis through insuring the 
      maintenance of centrosome and spindle assembly. Thus, the role of VPS4B in cancer 
      biology remains under debate. In this study, we analyzed the clinical 
      significance of VPS4B in NSCLC. The expression of VPS4B was evaluated by Western 
      blot in 8 paired fresh NSCLC tissues and immunohistochemistry on 105 
      paraffin-embedded slices. VPS4B was highly expressed in NSCLC and significantly 
      associated with NSCLCs tumor size, histological differentiation, clinical stage 
      and Ki-67. Besides, high VPS4B expression was an independent prognostic factor 
      for NSCLC patients' poor survival. To determine whether VPS4B could regulate the 
      proliferation of NSCLC cells, we knocked down the expression of VPS4B and 
      analyzed the proliferation of A549 NSCLC cells using Western blot, CCK8 and flow 
      cytometry assays, which together indicated that loss of VPS4B could inhibit cell 
      cycle progress. These data suggest that VPS4B may promote the progression of 
      NSCLC and be a biotarget for NSCLCs therapy.
FAU - Liu, Yifei
AU  - Liu Y
AD  - Department of Pathology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu, China.
FAU - Lv, Liting
AU  - Lv L
FAU - Xue, Qun
AU  - Xue Q
FAU - Wan, Chunhua
AU  - Wan C
FAU - Ni, Tingting
AU  - Ni T
FAU - Chen, Buyou
AU  - Chen B
FAU - Liu, Yanhua
AU  - Liu Y
FAU - Zhou, Yiqun
AU  - Zhou Y
FAU - Ni, Runzhou
AU  - Ni R
FAU - Mao, Guoxin
AU  - Mao G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130605
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Ki-67 Antigen)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities)
RN  - EC 3.6.4.6 (VPS4B protein, human)
SB  - IM
MH  - ATPases Associated with Diverse Cellular Activities
MH  - Adenosine Triphosphatases/*metabolism
MH  - Adult
MH  - Aged
MH  - Carcinoma, Non-Small-Cell Lung/*enzymology/*pathology
MH  - Cell Cycle
MH  - *Cell Division
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - *Disease Progression
MH  - Endosomal Sorting Complexes Required for Transport/*metabolism
MH  - Female
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Immunohistochemistry
MH  - Ki-67 Antigen/metabolism
MH  - Lung Neoplasms/*enzymology/*pathology
MH  - Male
MH  - Middle Aged
MH  - Proportional Hazards Models
MH  - Survival Analysis
EDAT- 2013/06/06 06:00
MHDA- 2014/02/13 06:00
CRDT- 2013/06/06 06:00
PHST- 2013/04/17 00:00 [received]
PHST- 2013/05/23 00:00 [accepted]
PHST- 2013/06/06 06:00 [entrez]
PHST- 2013/06/06 06:00 [pubmed]
PHST- 2014/02/13 06:00 [medline]
AID - 10.1007/s11010-013-1699-2 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2013 Sep;381(1-2):163-71. doi: 10.1007/s11010-013-1699-2. Epub 
      2013 Jun 5.