PMID- 23740575
OWN - NLM
STAT- MEDLINE
DCOM- 20131230
LR  - 20211021
IS  - 2046-2441 (Electronic)
IS  - 2046-2441 (Linking)
VI  - 3
IP  - 6
DP  - 2013 Jun 5
TI  - Hypoxia-induced invadopodia formation: a role for beta-PIX.
PG  - 120159
LID - 10.1098/rsob.120159 [doi]
LID - 120159
AB  - During tumour progression, oxygen tension in the microenvironment surrounding 
      tumour cells is reduced, resulting in hypoxia. It is well established that cancer 
      cells resist the negative effects of hypoxia by inducing angiogenesis 
      predominantly via the activity of transcription factor hypoxia-inducible factor-1 
      (HIF-1). However, more recently HIF-1alpha has also been linked to increased invasive 
      potential, although the molecular mechanisms remain to be defined. Invasive 
      cancer cells are thought to employ membrane protrusions, termed invadopodia, to 
      achieve matrix degradation. While many invadopodia components have been 
      identified, signalling pathways that link extracellular stimuli to invadopodia 
      formation remain largely unknown. Indeed, the relationship between invadopodia 
      formation and HIF-1alpha has not been explored. We now report that HIF-1alpha is a driver 
      of invadopodia formation. Furthermore, we have identified an important, direct 
      and novel link between the Rho family activator beta-PIX, HIF-1alpha and invadopodia 
      formation. Indeed, we find that beta-PIX expression is essential for invadopodia 
      formation. In conclusion, we identify a new HIF-1alpha mechanistic pathway and 
      suggest that beta-PIX is a novel downstream signalling mediator during invadopodia 
      formation.
FAU - Md Hashim, Nur Fariesha
AU  - Md Hashim NF
AD  - Division of Cancer Studies, King's College London, London SE1 1UL, UK.
FAU - Nicholas, Nicole S
AU  - Nicholas NS
FAU - Dart, Anna E
AU  - Dart AE
FAU - Kiriakidis, Serafim
AU  - Kiriakidis S
FAU - Paleolog, Ewa
AU  - Paleolog E
FAU - Wells, Claire M
AU  - Wells CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130605
PL  - England
TA  - Open Biol
JT  - Open biology
JID - 101580419
RN  - 0 (ARHGEF7 protein, human)
RN  - 0 (Amino Acids, Dicarboxylic)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Rho Guanine Nucleotide Exchange Factors)
RN  - VVW38EB8YS (oxalylglycine)
SB  - IM
MH  - Amino Acids, Dicarboxylic/pharmacology
MH  - *Cell Hypoxia
MH  - Cell Line, Tumor
MH  - HEK293 Cells
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/metabolism
MH  - RNA Interference
MH  - RNA, Messenger/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Rho Guanine Nucleotide Exchange Factors/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Up-Regulation/drug effects
PMC - PMC3718326
OTO - NOTNLM
OT  - hypoxia
OT  - invadopodia
OT  - beta-PIX
EDAT- 2013/06/07 06:00
MHDA- 2014/01/01 06:00
PMCR- 2013/06/01
CRDT- 2013/06/07 06:00
PHST- 2013/06/07 06:00 [entrez]
PHST- 2013/06/07 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
PHST- 2013/06/01 00:00 [pmc-release]
AID - rsob.120159 [pii]
AID - rsob120159 [pii]
AID - 10.1098/rsob.120159 [doi]
PST - epublish
SO  - Open Biol. 2013 Jun 5;3(6):120159. doi: 10.1098/rsob.120159.