PMID- 23742986
OWN - NLM
STAT- MEDLINE
DCOM- 20140219
LR  - 20130715
IS  - 1435-8115 (Electronic)
IS  - 1431-9276 (Linking)
VI  - 19
IP  - 4
DP  - 2013 Aug
TI  - Zyxin regulates cell migration and differentiation in EMT during chicken AV valve 
      morphogenesis.
PG  - 842-54
LID - 10.1017/S1431927613001633 [doi]
AB  - During heart valve development, epithelial-mesenchymal transformation (EMT) is a 
      key process for valve formation. EMT leads to the generation of mesenchymal cells 
      that will eventually become the interstitial cells (fibroblasts) of the mature 
      valve. During EMT, cell architecture and motility change markedly; significant 
      changes are also observed in various signaling pathways. Here we systematically 
      examined the expression, localization, and function of zyxin, a focal adhesion 
      protein, in EMT during atrioventricular (AV) valve morphogenesis. Expression and 
      localization studies showed that zyxin was expressed in the AV canal region 
      during crucial stages of valve development. An in vitro 3D collagen gel culture 
      system was used to determine zyxin function either after siRNA gene knockdown or 
      after overexpression. Our studies revealed that zyxin overexpression inhibited 
      endocardial cell migration and cell differentiation and also led to a decrease in 
      the number of migrating mesenchymal cells. Moreover, correlative cytoskeletal 
      changes were apparent in response to both overexpression and knockdown 
      treatments. Thus, zyxin appears to play a role as a regulator of cell migration 
      and differentiation during EMT in chicken AV valve formation.
FAU - Li, Na
AU  - Li N
AD  - Department of Cell Biology and Anatomy, School of Medicine, University of South 
      Carolina, Columbia, SC 29209, USA.
FAU - Goodwin, Richard L
AU  - Goodwin RL
FAU - Potts, Jay D
AU  - Potts JD
LA  - eng
GR  - K02HL086901/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130607
PL  - England
TA  - Microsc Microanal
JT  - Microscopy and microanalysis : the official journal of Microscopy Society of 
      America, Microbeam Analysis Society, Microscopical Society of Canada
JID - 9712707
RN  - 0 (Zyxin)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - *Cell Movement
MH  - Chick Embryo
MH  - *Gene Expression
MH  - Gene Silencing
MH  - Heart Valves/*embryology
MH  - Morphogenesis
MH  - Zyxin/*biosynthesis
EDAT- 2013/06/08 06:00
MHDA- 2014/02/20 06:00
CRDT- 2013/06/08 06:00
PHST- 2013/06/08 06:00 [entrez]
PHST- 2013/06/08 06:00 [pubmed]
PHST- 2014/02/20 06:00 [medline]
AID - S1431927613001633 [pii]
AID - 10.1017/S1431927613001633 [doi]
PST - ppublish
SO  - Microsc Microanal. 2013 Aug;19(4):842-54. doi: 10.1017/S1431927613001633. Epub 
      2013 Jun 7.