PMID- 23745742
OWN - NLM
STAT- MEDLINE
DCOM- 20140421
LR  - 20220408
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 47
IP  - 13
DP  - 2013 Jul 2
TI  - Effects of surface charge and hydrophobicity on anodic biofilm formation, 
      community composition, and current generation in bioelectrochemical systems.
PG  - 7563-70
LID - 10.1021/es400901u [doi]
AB  - The focus of this study was to investigate the effects of surface charge and 
      surface hydrophobicity on anodic biofilm formation, biofilm community 
      composition, and current generation in bioelectrochemical systems (BESs). Glassy 
      carbon surfaces were modified with -OH, -CH3, -SO3(-), or -N(+)(CH3)3 functional 
      groups by electrochemical reduction of aryl diazonium salts and then used as 
      anodes with poised potential of -0.2 V (vs Ag/AgCl). The average startup times 
      and final current densities for the -N(+)(CH3)3, -OH, -SO3(-), and -CH3, 
      electrodes were (23 d, 0.204 mA/cm(2)), (25.4 d, 0.149 mA/cm(2)), (25.9 d, 0.114 
      mA/cm(2)), and (37.2 d, 0.048 mA/cm(2)), respectively. Biofilms on different 
      surfaces were analyzed by nonturnover cyclic voltammetry (CV), fluorescence in 
      situ hybridization (FISH), and 16S rRNA gene amplicon pyrosequencing. The results 
      demonstrated that 1) differences in the maximum current output between surface 
      modifications was correlated with biomass quantity, and 2) all biofilms were 
      dominated by Geobacter populations, but the composition of -CH3-associated 
      biofilms differed from those formed on surfaces with different chemical 
      modification. This study shows that anode surface charge and hydrophobicity 
      influences biofilm development and can lead to significant differences in BESs 
      performance. Positively charged and hydrophilic surfaces were more selective to 
      electroactive microbes (e.g. Geobacter) and more conducive for electroactive 
      biofilm formation.
FAU - Guo, Kun
AU  - Guo K
AD  - Centre for Microbial Electrosynthesis, The University of Queensland, Brisbane, 
      QLD 4072, Australia.
FAU - Freguia, Stefano
AU  - Freguia S
FAU - Dennis, Paul G
AU  - Dennis PG
FAU - Chen, Xin
AU  - Chen X
FAU - Donose, Bogdan C
AU  - Donose BC
FAU - Keller, Jurg
AU  - Keller J
FAU - Gooding, J Justin
AU  - Gooding JJ
FAU - Rabaey, Korneel
AU  - Rabaey K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130619
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - *Bioelectric Energy Sources
MH  - *Biofilms
MH  - Electrodes/microbiology
MH  - Geobacter/*physiology
MH  - Hydrophobic and Hydrophilic Interactions
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics
MH  - Surface Properties
EDAT- 2013/06/12 06:00
MHDA- 2014/04/22 06:00
CRDT- 2013/06/11 06:00
PHST- 2013/06/11 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2014/04/22 06:00 [medline]
AID - 10.1021/es400901u [doi]
PST - ppublish
SO  - Environ Sci Technol. 2013 Jul 2;47(13):7563-70. doi: 10.1021/es400901u. Epub 2013 
      Jun 19.