PMID- 23746952
OWN - NLM
STAT- MEDLINE
DCOM- 20140312
LR  - 20171116
IS  - 1618-095X (Electronic)
IS  - 0944-7113 (Linking)
VI  - 20
IP  - 12
DP  - 2013 Sep 15
TI  - Effects of imperatorin, the active component from Radix Angelicae (Baizhi), on 
      the blood pressure and oxidative stress in 2K,1C hypertensive rats.
PG  - 1048-54
LID - S0944-7113(13)00199-2 [pii]
LID - 10.1016/j.phymed.2013.04.021 [doi]
AB  - The 2-kidney, 1-clip (2K,1C) model of hypertension was used to investigate the 
      potential antihypertensive and antioxidant effect of imperatorin extracted from 
      the root of radix angelicae. After 10 weeks treatment of imperatorin, mean blood 
      pressure (MBP) of 2K,1C hypertensive rats was obtained, and superoxide dismutase 
      (SOD), nitric oxide (NO) and nitric oxide synthase (NOS) were measured. 
      Malondialdehyde (MDA) and glutathione (GSH) levels, catalase (CATA), xanthine 
      oxidase (XOD), angiotensinII (Ang II) and endothelin (ET) levels of kidney were 
      evaluated with commercial kits. Nicotinamide adenine dinucleotidephosphate 
      (NADPH) oxidase subunits of the renal cortial tissues were determined by RT-PCR 
      and Western blot. 8-Iso-prostaglandin F2alpha (8-iso-PGF2alpha) of 24h urinary excretion 
      was also measured by ELISA. MBP was significantly reduced by treatment with IMP 
      (6.25, 12.5 and 25 mg/kg/day, i.g.) in 2K,1C hypertensive rats. Meanwhile, we 
      found that renal CATA and XOD activities, GSH levels, plasma NO and NOS contents 
      were significantly increased in IMP-treated groups. Plasma ET, renal Ang II 
      levels, MDA and the 24h urinary excretion of 8-iso-PGF2alpha in the IMP treated group 
      were lower than control SD group. After that, we found the mRNA expressions and 
      protein levels of NADPH oxidase subunits in the clipped kidney were markedly 
      reduced after IMP treated in 2K,1C hypertensive rats. IMP showed antihypertensive 
      and antioxidant effects in the renal injury of renovascular hypertensive rats, 
      suggesting that IMP could be of therapeutic use in preventing renal injury 
      related hypertension.
CI  - Copyright (c) 2013 Elsevier GmbH. All rights reserved.
FAU - Cao, Yan-Jun
AU  - Cao YJ
AD  - School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.
FAU - He, Xu
AU  - He X
FAU - Wang, Nan
AU  - Wang N
FAU - He, Lang-Chong
AU  - He LC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130606
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Antihypertensive Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Furocoumarins)
RN  - 0 (Membrane Glycoproteins)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.6.3.- (Cybb protein, rat)
RN  - EC 1.6.3.- (NADPH Oxidase 2)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.1 (neutrophil cytosolic factor 1)
RN  - K713N25C78 (imperatorin)
SB  - IM
MH  - Angelica/*chemistry
MH  - Animals
MH  - Antihypertensive Agents/administration & dosage/isolation & 
      purification/*pharmacology
MH  - Antioxidants/analysis/*metabolism
MH  - Blood Pressure/drug effects
MH  - Disease Models, Animal
MH  - Furocoumarins/administration & dosage/isolation & purification/*pharmacology
MH  - Hypertension, Renovascular/*drug therapy/metabolism/physiopathology
MH  - Kidney/drug effects/metabolism/physiopathology
MH  - Male
MH  - Membrane Glycoproteins/drug effects/metabolism
MH  - NADPH Oxidase 2
MH  - NADPH Oxidases/drug effects/genetics/metabolism
MH  - Nitric Oxide/analysis/metabolism
MH  - Oxidative Stress/drug effects
MH  - Plant Roots/chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - 2K,1C hypertension
OT  - 8-Iso-PGF2alpha
OT  - 8-iso-prostaglandin F2alpha
OT  - AngII
OT  - Angelica dahurica
OT  - BP
OT  - CATA
OT  - ET
OT  - GSH
OT  - Imperatorin (IMP)
OT  - MBP
OT  - MDA
OT  - NADPH
OT  - NADPH oxidase
OT  - NO
OT  - NOS
OT  - Oxidase stress
OT  - RHR
OT  - SD rats
OT  - SOD
OT  - Sprague-Dawley rats.
OT  - XOD
OT  - angiotensinII
OT  - bloodpressure
OT  - catalase
OT  - endothelin
OT  - glutathione
OT  - malondialdehyde
OT  - mean blood pressure
OT  - nicotinamide adenine dinucleotidephosphate
OT  - nitric oxide
OT  - nitric oxide synthase
OT  - renovascular-hypertensive rats, two kidneys one clip model
OT  - superoxide dismutase
OT  - xanthine oxidase
EDAT- 2013/06/12 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/06/11 06:00
PHST- 2012/12/20 00:00 [received]
PHST- 2013/03/19 00:00 [revised]
PHST- 2013/04/23 00:00 [accepted]
PHST- 2013/06/11 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - S0944-7113(13)00199-2 [pii]
AID - 10.1016/j.phymed.2013.04.021 [doi]
PST - ppublish
SO  - Phytomedicine. 2013 Sep 15;20(12):1048-54. doi: 10.1016/j.phymed.2013.04.021. 
      Epub 2013 Jun 6.