PMID- 23747599
OWN - NLM
STAT- MEDLINE
DCOM- 20131028
LR  - 20130812
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Linking)
VI  - 381
IP  - 1
DP  - 2013 Sep 1
TI  - CaMK-II activation is essential for zebrafish inner ear development and acts 
      through Delta-Notch signaling.
PG  - 179-88
LID - S0012-1606(13)00310-2 [pii]
LID - 10.1016/j.ydbio.2013.05.028 [doi]
AB  - Zebrafish inner ear development is characterized by the crystallization of 
      otoliths onto immotile kinocilia that protrude from sensory "hair" cells. The 
      stereotypical formation of these sensory structures is dependent on the 
      expression of key patterning genes and on Ca2+ signals. One potential target of 
      Ca2+ signaling in the inner ear is the type II Ca2+/calmodulin-dependent protein 
      kinase (CaMK-II), which is preferentially activated in hair cells, with intense 
      activation at the base of kinocilia. In zebrafish, CaMK-II is encoded by seven 
      genes; the expression of one of these genes (camk2g1) is enriched in hair cells. 
      The suppression of camk2g1 expression by antisense morpholino oligonucleotides or 
      inhibition of CaMK-II activation by the pharmacological antagonist, KN-93, 
      results in aberrant otolith formation without preventing cilia formation. In 
      fact, CaMK-II suppression results in additional ciliated hair cells and altered 
      levels of Delta-Notch signaling members. DeltaA and deltaD transcripts are 
      increased and DeltaD protein accumulates in hair cells of CaMK-II morphants, 
      indicative of defective recycling and/or exocytosis. Our findings indicate that 
      CaMK-II plays a critical role in the developing ear, influencing cell 
      differentiation through extranuclear effects on Delta-Notch signaling. Continued 
      expression and activation of CaMK-II in maculae and cristae in older embryos 
      suggests continued roles in auditory sensory maturation and transduction.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Rothschild, Sarah C
AU  - Rothschild SC
AD  - Department of Biology, Virginia Commonwealth University, Richmond, VA, USA.
FAU - Lahvic, Jamie
AU  - Lahvic J
FAU - Francescatto, Ludmila
AU  - Francescatto L
FAU - McLeod, Jamie J A
AU  - McLeod JJ
FAU - Burgess, Shawn M
AU  - Burgess SM
FAU - Tombes, Robert M
AU  - Tombes RM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130607
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Oligonucleotides)
RN  - 0 (Receptors, Notch)
RN  - 0 (delta protein)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
SB  - IM
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/*metabolism
MH  - Cilia/metabolism
MH  - Ear, Inner/*embryology
MH  - Enzyme Activation
MH  - Exocytosis
MH  - *Gene Expression Regulation, Developmental
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Membrane Proteins/*metabolism
MH  - Oligonucleotides/metabolism
MH  - Phosphorylation
MH  - Receptors, Notch/*metabolism
MH  - Signal Transduction
MH  - Zebrafish/*embryology
OTO - NOTNLM
OT  - CaMK-II
OT  - Cilia
OT  - Delta-notch
OT  - Inner ear
OT  - Otolith
EDAT- 2013/06/12 06:00
MHDA- 2013/10/29 06:00
CRDT- 2013/06/11 06:00
PHST- 2013/02/17 00:00 [received]
PHST- 2013/05/28 00:00 [revised]
PHST- 2013/05/29 00:00 [accepted]
PHST- 2013/06/11 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2013/10/29 06:00 [medline]
AID - S0012-1606(13)00310-2 [pii]
AID - 10.1016/j.ydbio.2013.05.028 [doi]
PST - ppublish
SO  - Dev Biol. 2013 Sep 1;381(1):179-88. doi: 10.1016/j.ydbio.2013.05.028. Epub 2013 
      Jun 7.