PMID- 23747724
OWN - NLM
STAT- MEDLINE
DCOM- 20130909
LR  - 20130708
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 436
IP  - 3
DP  - 2013 Jul 5
TI  - Effects of chemokine (C-C motif) ligand 1 on microglial function.
PG  - 455-61
LID - S0006-291X(13)00944-3 [pii]
LID - 10.1016/j.bbrc.2013.05.126 [doi]
AB  - Microglia, which constitute the resident macrophages of the central nervous 
      system (CNS), are generally considered as the primary immune cells in the brain 
      and spinal cord. Microglial cells respond to various factors which are produced 
      following nerve injury of multiple aetiologies and contribute to the development 
      of neuronal disease. Chemokine (C-C motif) ligand 1 (CCL-1), a well-characterized 
      chemokine secreted by activated T cells, has been shown to play an important role 
      in neuropathic pain induced by nerve injury and is also produced in various cell 
      types in the CNS, especially in dorsal root ganglia (DRG). However, the role of 
      CCL-1 in the CNS and the effects on microglia remains unclear. Here we showed the 
      multiple effects of CCL-1 on microglia. We first showed that CCR-8, a specific 
      receptor for CCL-1, was expressed on primary cultured microglia, as well as on 
      astrocytes and neurons, and was upregulated in the presence of CCL-1. CCL-1 at 
      concentration of 1 ng/ml induced chemotaxis, increased motility at a higher 
      concentration (100 ng/ml), and increased proliferation and phagocytosis of 
      cultured microglia. CCL-1 also activated microglia morphologically, promoted mRNA 
      levels for brain-derived neurotrophic factor (BDNF) and IL-6, and increased the 
      release of nitrite from microglia. These indicate that CCL-1 has a role as a 
      mediator in neuron-glia interaction, which may contribute to the development of 
      neurological diseases, especially in neuropathic pain.
CI  - Copyright (c) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Akimoto, Nozomi
AU  - Akimoto N
AD  - Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu 
      University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
FAU - Ifuku, Masataka
AU  - Ifuku M
FAU - Mori, Yuki
AU  - Mori Y
FAU - Noda, Mami
AU  - Noda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130606
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ccl1 protein, mouse)
RN  - 0 (Chemokine CCL1)
RN  - 0 (Interleukin-6)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Nitrites)
RN  - 0 (RNA, Messenger)
RN  - 0 (interleukin-6, mouse)
SB  - IM
MH  - Animals
MH  - Astrocytes/drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Count
MH  - Cell Proliferation
MH  - Cell Shape/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL1/*metabolism/pharmacology
MH  - Chemotaxis
MH  - *Gene Expression Regulation
MH  - Interleukin-6/genetics/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Mice
MH  - Microglia/drug effects/*metabolism
MH  - Neurons/drug effects/metabolism
MH  - Nitrites/metabolism
MH  - Phagocytosis
MH  - Primary Cell Culture
MH  - RNA, Messenger/genetics/metabolism
OTO - NOTNLM
OT  - 4',6'-diamidino-2-phenylindole dihydrochloride
OT  - BDNF
OT  - CCL-1
OT  - CCL-2
OT  - CCL-21
OT  - CCR-8
OT  - CNS
OT  - CX3CL-1
OT  - Chemotaxis
OT  - Cytokines
OT  - DAPI
OT  - DMEM
OT  - DRG
OT  - FBS
OT  - GABA
OT  - GFAP
OT  - IL-1beta
OT  - IL-6
OT  - Iba1
OT  - MAP2
OT  - Microglia
OT  - NMDA receptor 2B
OT  - NO
OT  - NR2B
OT  - PBS
OT  - Phagocytosis
OT  - Proliferation
OT  - RT-PCR
OT  - TCA-3
OT  - TNF-alpha
OT  - base pair
OT  - bp
OT  - brain-derived neurotrophic factor
OT  - cDNA
OT  - central nervous system
OT  - chemokine (CX3C motif) ligand 1
OT  - chemokine (C-C motif) ligand 1
OT  - chemokine (C-C motif) ligand 2
OT  - chemokine (C-C motif) ligand 21
OT  - chemokine (C-C motif) receptor 8
OT  - complementary DNA
OT  - dorsal root ganglia
OT  - dulbecco's modified eagle medium
OT  - fetal bovine serum
OT  - glial fibrillary acid protein
OT  - interleukin 1beta
OT  - interleukin 6
OT  - ionized calcium-binding adapter molecule 1
OT  - mRNA
OT  - messenger RNA
OT  - microtubule-associated protein 2
OT  - nitric oxide
OT  - phosphate buffered saline
OT  - reverse transcription polymerase chain reaction
OT  - thymus-derived chemotactic agent 3
OT  - tumour necrosis factor-alpha
OT  - gamma-amino butyric acid
EDAT- 2013/06/12 06:00
MHDA- 2013/09/10 06:00
CRDT- 2013/06/11 06:00
PHST- 2013/05/23 00:00 [received]
PHST- 2013/05/29 00:00 [accepted]
PHST- 2013/06/11 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2013/09/10 06:00 [medline]
AID - S0006-291X(13)00944-3 [pii]
AID - 10.1016/j.bbrc.2013.05.126 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2013 Jul 5;436(3):455-61. doi: 
      10.1016/j.bbrc.2013.05.126. Epub 2013 Jun 6.