PMID- 23748502
OWN - NLM
STAT- MEDLINE
DCOM- 20140213
LR  - 20220318
IS  - 1546-1696 (Electronic)
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 31
IP  - 7
DP  - 2013 Jul
TI  - Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell 
      epitope mapping and characterization.
PG  - 623-9
LID - 10.1038/nbt.2593 [doi]
AB  - It is currently not possible to predict which epitopes will be recognized by T 
      cells in different individuals. This is a barrier to the thorough analysis and 
      understanding of T-cell responses after vaccination or infection. Here, by 
      combining mass cytometry with combinatorial peptide-MHC tetramer staining, we 
      have developed a method allowing the rapid and simultaneous identification and 
      characterization of T cells specific for many epitopes. We use this to screen up 
      to 109 different peptide-MHC tetramers in a single human blood sample, while 
      still retaining at least 23 labels to analyze other markers of T-cell phenotype 
      and function. Among 77 candidate rotavirus epitopes, we identified six T-cell 
      epitopes restricted to human leukocyte antigen (HLA)-A*0201 in the blood of 
      healthy individuals. T cells specific for epitopes in the rotavirus VP3 protein 
      displayed a distinct phenotype and were present at high frequencies in intestinal 
      epithelium. This approach should be useful for the comprehensive analysis of 
      T-cell responses to infectious diseases or vaccines.
FAU - Newell, Evan W
AU  - Newell EW
AD  - Agency for Science, Technology and Research, Singapore Immunology Network, 
      Singapore. evan_newell@immunol.a-star.edu.sg
FAU - Sigal, Natalia
AU  - Sigal N
FAU - Nair, Nitya
AU  - Nair N
FAU - Kidd, Brian A
AU  - Kidd BA
FAU - Greenberg, Harry B
AU  - Greenberg HB
FAU - Davis, Mark M
AU  - Davis MM
LA  - eng
GR  - R01 AI021362/AI/NIAID NIH HHS/United States
GR  - U19-AI090019/AI/NIAID NIH HHS/United States
GR  - U19 AI090019/AI/NIAID NIH HHS/United States
GR  - U19-AI057229/AI/NIAID NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - U19 AI057229/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130609
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptides)
SB  - IM
CIN - Nat Biotechnol. 2013 Jul;31(7):609-10. PMID: 23839145
MH  - Antigens, Viral/immunology
MH  - *Epitope Mapping
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Flow Cytometry
MH  - HLA-A Antigens/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Histocompatibility Antigens Class II/immunology
MH  - Humans
MH  - Mass Spectrometry
MH  - Peptides/chemistry/*immunology
MH  - Rotavirus/immunology/metabolism
MH  - T-Lymphocytes
PMC - PMC3796952
MID - NIHMS501485
EDAT- 2013/06/12 06:00
MHDA- 2014/02/14 06:00
PMCR- 2013/10/15
CRDT- 2013/06/11 06:00
PHST- 2013/02/07 00:00 [received]
PHST- 2013/04/22 00:00 [accepted]
PHST- 2013/06/11 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2014/02/14 06:00 [medline]
PHST- 2013/10/15 00:00 [pmc-release]
AID - nbt.2593 [pii]
AID - 10.1038/nbt.2593 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2013 Jul;31(7):623-9. doi: 10.1038/nbt.2593. Epub 2013 Jun 9.