PMID- 23749487
OWN - NLM
STAT- MEDLINE
DCOM- 20131126
LR  - 20211021
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 34
IP  - 5
DP  - 2013 Oct
TI  - Chromosomal imbalances exclusively detected in invasive front area are associated 
      with poor outcome in laryngeal carcinomas from different anatomical sites.
PG  - 3015-26
LID - 10.1007/s13277-013-0866-0 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is a malignant neoplasm exhibiting 
      aggressive phenotype, high recurrence rate, and risk of developing second primary 
      tumors. Current evidence suggests that cells in the invasive front of carcinomas 
      have different molecular profiles compared to those in superficial areas. This 
      study aimed to identify candidate genes in the invasive front and superficial 
      cells from laryngeal carcinomas that would be useful as molecular markers. 
      Invasive front and tumor surface cells of 32 LSCC were evaluated by 
      high-resolution comparative genomic hybridization. Both CCND1 copy number gains 
      and cyclin D1 protein expression were evaluated to confirm gains of 11q13.3. 
      Losses of 3q26.2-q29 and 18q23 were confirmed by loss of heterozygosity analysis. 
      The most frequent chromosomal alterations observed only in invasive front cells 
      involved gains of 1p, 4q, and 9p and losses of 3p, 11p, 12p, 13q, 17q, 18p, 19q, 
      20q, 21q, and Xp. Gains of 11q13 were detected in both components from glottis 
      and supraglottis but only in invasive front cells from transglottic tumors. 
      Fluorescence in situ hybridization confirmed gains of CCND1/CPE11 in a subset of 
      cases. In supraglottic tumors, cyclin D1 positivity was associated with distant 
      metastasis (P = 0.0018) and with decreased disease-free survival (P = 0.042). 
      Loss of heterozygosity at 3q26.2 and 18q23 were associated with lymph node 
      involvement (P = 0.055) and worsened prognosis, respectively. In conclusion, this 
      study revealed regions that could be targeted in the search for molecular markers 
      in LSCC. Cyclin D1 may be useful as a prognostic marker in supraglottic tumors.
FAU - Ambrosio, Eliane Papa
AU  - Ambrosio EP
AD  - Institute of Biosciences, UNESP-Sao Paulo State University, Botucatu, SP, Brazil.
FAU - Silveira, Cassia Gisele Terrassani
AU  - Silveira CG
FAU - Drigo, Sandra Aparecida
AU  - Drigo SA
FAU - Sacomano, Vivian de Souza
AU  - Sacomano Vde S
FAU - Molck, Miriam Coelho
AU  - Molck MC
FAU - Rocha, Rafael Malagoli
AU  - Rocha RM
FAU - Domingues, Maria Aparecida Custodio
AU  - Domingues MA
FAU - Soares, Fernando Augusto
AU  - Soares FA
FAU - Kowalski, Luiz Paulo
AU  - Kowalski LP
FAU - Rogatto, Silvia Regina
AU  - Rogatto SR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130608
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (CCND1 protein, human)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/secondary
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human
MH  - Comparative Genomic Hybridization
MH  - Cyclin D1/metabolism
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Laser Capture Microdissection
MH  - Loss of Heterozygosity
MH  - Lymphatic Metastasis
MH  - Male
MH  - Microsatellite Instability
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Tissue Array Analysis
EDAT- 2013/06/12 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/06/11 06:00
PHST- 2013/02/27 00:00 [received]
PHST- 2013/05/13 00:00 [accepted]
PHST- 2013/06/11 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1007/s13277-013-0866-0 [doi]
PST - ppublish
SO  - Tumour Biol. 2013 Oct;34(5):3015-26. doi: 10.1007/s13277-013-0866-0. Epub 2013 
      Jun 8.