PMID- 23749613
OWN - NLM
STAT- MEDLINE
DCOM- 20140114
LR  - 20220318
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 54
IP  - 3
DP  - 2013 Aug
TI  - Characterization of a 5HS-7DS.7DL wheat-barley translocation line and physical 
      mapping of the 7D chromosome using SSR markers.
PG  - 251-8
LID - 10.1007/s13353-013-0152-2 [doi]
AB  - A spontaneous wheat-barley translocation line was previously detected in the 
      progenies of the Mv9kr1 x 'Igri' wheat-barley hybrid and the translocation was 
      identified as 5HS-7DS.7DL. Multicolor genomic in situ hybridization (mcGISH) with 
      D and H genomic DNA probes and three-color fluorescence in situ hybridization 
      (FISH) with repetitive DNA probes (Afa-family, pSc119.2, and pTa71) were 
      performed to characterize the rearranged chromosome. The effect of 5HS and the 
      deleted 7DS fragment on the morphological traits (plant height, fertility, yield, 
      and spike characteristics) of wheat was assessed. Despite the non-compensating 
      nature of the translocation, the plants showed good viability. The aim of the 
      study was to physically localize SSR markers to the telomeric and subtelomeric 
      regions of the 7DS chromosome arm. Of the 45 microsatellite markers analyzed, ten 
      (Xbarc0184, Xwmc0506, Xgdm0130, Xgwm0735, Xgwm1258, Xgwm1123, Xgwm1250, Xgwm1055, 
      Xgwm1220, and Xgwm0635) failed to amplify any 7DS-specific fragments, signaling 
      the elimination of a short chromosome segment in the telomeric region. The 
      breakpoint of the 5HS-7DS.7DL translocation appeared to be more distal than that 
      of reported deletion lines, which provides a new physical landmark for future 
      deletion mapping studies.
FAU - Kruppa, Klaudia
AU  - Kruppa K
AD  - Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of 
      Sciences, Brunszvik 2, 2462, Martonvasar, Hungary.
FAU - Sepsi, Adel
AU  - Sepsi A
FAU - Szakacs, Eva
AU  - Szakacs E
FAU - Roder, Marion S
AU  - Roder MS
FAU - Molnar-Lang, Marta
AU  - Molnar-Lang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130608
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosomes, Plant
MH  - DNA Probes/genetics
MH  - Gene Deletion
MH  - *Genes, Plant
MH  - Genetic Markers
MH  - Hordeum/*genetics
MH  - In Situ Hybridization
MH  - In Situ Hybridization, Fluorescence
MH  - Microsatellite Repeats/genetics
MH  - Phenotype
MH  - Physical Chromosome Mapping
MH  - *Translocation, Genetic
MH  - Triticum/*genetics
EDAT- 2013/06/12 06:00
MHDA- 2014/01/15 06:00
CRDT- 2013/06/11 06:00
PHST- 2013/03/13 00:00 [received]
PHST- 2013/05/13 00:00 [accepted]
PHST- 2013/05/09 00:00 [revised]
PHST- 2013/06/11 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2014/01/15 06:00 [medline]
AID - 10.1007/s13353-013-0152-2 [doi]
PST - ppublish
SO  - J Appl Genet. 2013 Aug;54(3):251-8. doi: 10.1007/s13353-013-0152-2. Epub 2013 Jun 
      8.