PMID- 23749949
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20220317
IS  - 1460-2091 (Electronic)
IS  - 0305-7453 (Linking)
VI  - 68
IP  - 10
DP  - 2013 Oct
TI  - Doxycycline suppresses Chlamydia pneumoniae-mediated increases in ongoing 
      immunoglobulin E and interleukin-4 responses by peripheral blood mononuclear 
      cells of patients with allergic asthma.
PG  - 2363-8
LID - 10.1093/jac/dkt179 [doi]
AB  - OBJECTIVES: Chlamydia pneumoniae, an obligate intracellular bacterium, has been 
      associated with asthma and the induction of immunoglobulin E (IgE) responses. 
      Whereas tetracyclines have anti-chlamydial activity, their effect on human IgE 
      responses to C. pneumoniae has not been studied. METHODS: Peripheral blood 
      mononuclear cells (PBMCs) from serum IgE+ allergic asthmatic subjects (n = 11) 
      and healthy controls (n = 12) were infected with C. pneumoniae and cultured for 
      12 days with or without doxycycline (0.01-1.0 mg/L). IgE, interferon (IFN)-gamma and 
      interleukin (IL)-4 levels in supernatants were determined on days 1-12 
      post-infection, and C. pneumoniae DNA copy numbers in PBMC culture were measured 
      on day 2 (quantitative PCR). RESULTS: C. pneumoniae-infected PBMCs from allergic 
      asthmatic individuals had increased levels of IgE in supernatants compared with 
      uninfected PBMCs (520% on day 10 post-infection, P = 0.008). IgE levels in PBMC 
      cultures from controls were undetectable (<0.3 ng/mL). Increases in C. 
      pneumoniae-induced IgE in asthmatics correlated with those of C. 
      pneumoniae-induced IL-4 (r = 0.98; P < 0.001), but not with IFN-gamma. The addition 
      of doxycycline (1.0 mg/L) to the culture strongly suppressed the production of 
      IgE (>70%, P = 0.04) and IL-4 (75%, P = 0.018), but not IFN-gamma. The suppressive 
      effect on IL-4 production remained significant even at concentrations of 
      doxycycline that were subinhibitory (0.01 mg/L) for C. pneumoniae. In both 
      asthmatic participants and controls, no significant effect of doxycycline on DNA 
      copy numbers of C. pneumoniae was observed. CONCLUSIONS: Doxycycline suppressed 
      the C. pneumoniae-induced production of IgE and IL-4, but not IFN-gamma, in PBMCs 
      from IgE+ allergic asthmatic subjects. These findings resulted from the 
      immunomodulatory anti-allergic properties of tetracyclines.
FAU - Dzhindzhikhashvili, M S
AU  - Dzhindzhikhashvili MS
AD  - Center for Allergy and Asthma Research at SUNY Downstate, State University of New 
      York (SUNY) Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
FAU - Joks, Rauno
AU  - Joks R
FAU - Smith-Norowitz, Tamar
AU  - Smith-Norowitz T
FAU - Durkin, Helen G
AU  - Durkin HG
FAU - Chotikanatis, Kobkul
AU  - Chotikanatis K
FAU - Estrella, Eva
AU  - Estrella E
FAU - Hammerschlag, Margaret R
AU  - Hammerschlag MR
FAU - Kohlhoff, Stephan A
AU  - Kohlhoff SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130607
PL  - England
TA  - J Antimicrob Chemother
JT  - The Journal of antimicrobial chemotherapy
JID - 7513617
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (IL4 protein, human)
RN  - 0 (Immunologic Factors)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - N12000U13O (Doxycycline)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Anti-Bacterial Agents/*administration & dosage
MH  - Asthma/*drug therapy/immunology
MH  - Bacterial Load
MH  - Cells, Cultured
MH  - Chlamydophila Infections/*complications/microbiology
MH  - Chlamydophila pneumoniae/immunology
MH  - Doxycycline/*administration & dosage
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Immunologic Factors/*administration & dosage
MH  - Interleukin-4/*metabolism
MH  - Leukocytes, Mononuclear/immunology/microbiology
MH  - Male
MH  - Middle Aged
MH  - Young Adult
OTO - NOTNLM
OT  - cytokines
OT  - immunomodulation
OT  - inflammation
OT  - tetracyclines
EDAT- 2013/06/12 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/06/11 06:00
PHST- 2013/06/11 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - dkt179 [pii]
AID - 10.1093/jac/dkt179 [doi]
PST - ppublish
SO  - J Antimicrob Chemother. 2013 Oct;68(10):2363-8. doi: 10.1093/jac/dkt179. Epub 
      2013 Jun 7.