PMID- 23751182
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20191231
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 132
IP  - 1
DP  - 2013 Jul
TI  - Anti-thrombosis effect of LRRFIP1 shRNA lentivirus in a mouse model of deep vein 
      thrombosis.
PG  - 127-31
LID - S0049-3848(13)00205-3 [pii]
LID - 10.1016/j.thromres.2013.05.013 [doi]
AB  - INTRODUCTION: Deep vein thrombosis (DVT) is one of the common complications of 
      orthopedic surgery. Low molecular weight heparin (LMWH) is a usually used agent 
      for DVT, but it would increase the risk of bleeding. LRRFIP1 has been shown to 
      play an important role in the formation of thrombosis. Therefore, we investigated 
      the effect of LRRFIP1 shRNA lentivirus on DVT in mice. MATERIALS AND METHODS: 
      Lentiviral Vectors carrying LRRFIP1 shRNA were constructed and transfected into 
      cultured mouse bone marrow cells (BMCs). Male ICR mice were irradiated with a 
      single dose of 9.5 Gy and then were injected with different agents through the 
      tail vein. Stasis venous thrombosis was induced by inferior vena cava (IVC) 
      ligation. Mice were sacrificed on the 1st, 3rd and 7th day post operation and the 
      thrombi were removed, blotted the excess blood on it with filter paper and 
      immediately weighed. P-selectin and d-Dimer were determined by enzyme-linked 
      immunosorbent assay (ELISA). RESULTS: LRRFIP1 shRNA significantly suppressed the 
      expression of LRRFIP1 in the thrombi. In contrast, low molecular weight heparin 
      (LMWH) and negative shRNA exhibited little effect on the expression of LRRFIP1. 
      LRRFIP1 shRNA, LMWH and negative shRNA inhibited the thrombus formation in vivo 
      significantly. The plasma P-selectin and d-Dimer levels were significantly 
      increased after IVC ligation. LRRFIP1 shRNA significantly decreased the plasma 
      P-selectin and d-Dimer levels. However, LMWH and negative shRNA showed little 
      effects on the levels of plasma P-selectin and d-Dimer. CONCLUSION: LRRFIP1 shRNA 
      might represent a promising prevention strategy for DVT.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Yin, Xiang
AU  - Yin X
AD  - Department of orthopaedics, Institute of surgery research, Daping Hospital, Third 
      Military Medical University, Chongqing 400042, PR China.
FAU - Wu, Siyu
AU  - Wu S
FAU - Wang, Ziming
AU  - Wang Z
FAU - Wang, Yu
AU  - Wang Y
FAU - Du, Quanyin
AU  - Du Q
FAU - Wang, Aimin
AU  - Wang A
LA  - eng
PT  - Journal Article
DEP - 20130614
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Anticoagulants)
RN  - 0 (Fibrin Fibrinogen Degradation Products)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Lrrfip1 protein, mouse)
RN  - 0 (P-Selectin)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (fibrin fragment D)
SB  - IM
MH  - Animals
MH  - Anticoagulants/pharmacology/therapeutic use
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Fibrin Fibrinogen Degradation Products/analysis/metabolism
MH  - Genetic Vectors/genetics/*therapeutic use
MH  - Heparin, Low-Molecular-Weight/pharmacology/therapeutic use
MH  - Lentivirus/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - P-Selectin/blood/metabolism
MH  - Phosphoproteins/*genetics
MH  - *RNA Interference
MH  - RNA, Small Interfering/genetics/*therapeutic use
MH  - Transfection
MH  - Venous Thrombosis/blood/*genetics/metabolism/*therapy
OTO - NOTNLM
OT  - Anti-thrombosis
OT  - BMCs
OT  - DVT
OT  - Deep vein thrombosis
OT  - ELISA
OT  - GFP
OT  - IVC
OT  - LMWH
OT  - LRRFIP1
OT  - PE
OT  - THA
OT  - TKA
OT  - bone marrow cells
OT  - deep vein thrombosis
OT  - enzyme-linked immunosorbent assay
OT  - green fluorescent protein
OT  - inferior vena cava
OT  - leucine-rich repeat in Flightless-1 interaction protein 1
OT  - low molecular weight heparin
OT  - p-Selectin
OT  - pulmonary embolism
OT  - siRNA
OT  - small interfering RNA
OT  - total hip arthroplasty
OT  - total knee arthroplasty
EDAT- 2013/06/12 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/06/12 06:00
PHST- 2013/03/04 00:00 [received]
PHST- 2013/05/09 00:00 [revised]
PHST- 2013/05/10 00:00 [accepted]
PHST- 2013/06/12 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - S0049-3848(13)00205-3 [pii]
AID - 10.1016/j.thromres.2013.05.013 [doi]
PST - ppublish
SO  - Thromb Res. 2013 Jul;132(1):127-31. doi: 10.1016/j.thromres.2013.05.013. Epub 
      2013 Jun 14.