PMID- 23752352
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20130716
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 138
IP  - 16
DP  - 2013 Aug 21
TI  - Quantification of tumor necrosis factor-alpha and matrix metalloproteinases-3 in 
      synovial fluid by a fiber-optic particle plasmon resonance sensor.
PG  - 4599-606
LID - 10.1039/c3an00276d [doi]
AB  - The availability of techniques for sensitive detection of early stage 
      osteoarthritis is critical for improving patient health. This study illustrates 
      the feasibility of a fiber-optic particle plasmon resonance (FOPPR) sensor with 
      gold nanoparticles on the unclad region of optical fiber probes for analysis of 
      osteoarthritis biomarkers, tumor necrosis factor-alpha (TNF-alpha) and matrix 
      metalloproteinases-3 (MMP-3). Results show that the sensor can achieve a 
      refractive index resolution of 5.18 x 10(-)(7) RIU and limits of detection for TNF-alpha 
      and MMP-3 as low as 8.22 pg ml(-)(1) (0.48 pM) and 34.3 pg ml(-)(1) (1.56 pM), 
      respectively. Additionally, the FOPPR sensor shows a good correlation in 
      determining TNF-alpha and MMP-3 in synovial fluid with the clinically accepted 
      enzyme-linked immunosorbent assay (ELISA) method. Finally, given the FOPPR 
      sensor's nature of being low-cost, label-free, highly sensitive, real-time, 
      simple-to-operate, the FOPPR sensor could offer potential to monitor biomarkers 
      of various diseases, and provide an ideal technical tool for point-of-care 
      diagnostics.
FAU - Huang, Yi-Ching
AU  - Huang YC
AD  - Department of Chemistry and Biochemistry, National Chung Cheng University, 
      Chiayi, 621, Taiwan.
FAU - Chiang, Chang-Yue
AU  - Chiang CY
FAU - Li, Cheng-Hen
AU  - Li CH
FAU - Chang, Ting-Chou
AU  - Chang TC
FAU - Chiang, Chung-Sheng
AU  - Chiang CS
FAU - Chau, Lai-Kwan
AU  - Chau LK
FAU - Huang, Kuo-Wei
AU  - Huang KW
FAU - Wu, Chin-Wei
AU  - Wu CW
FAU - Wang, Shau-Chun
AU  - Wang SC
FAU - Lyu, Shaw-Ruey
AU  - Lyu SR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Feasibility Studies
MH  - Humans
MH  - Matrix Metalloproteinase 3/*analysis
MH  - *Optical Fibers
MH  - Osteoarthritis/diagnosis
MH  - Surface Plasmon Resonance/*methods
MH  - Synovial Fluid/*chemistry
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 2013/06/12 06:00
MHDA- 2014/04/23 06:00
CRDT- 2013/06/12 06:00
PHST- 2013/06/12 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
AID - 10.1039/c3an00276d [doi]
PST - ppublish
SO  - Analyst. 2013 Aug 21;138(16):4599-606. doi: 10.1039/c3an00276d.