PMID- 23752384
OWN - NLM
STAT- MEDLINE
DCOM- 20130910
LR  - 20130611
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 67
IP  - 11
DP  - 2013
TI  - The Microbial Database for Danish wastewater treatment plants with nutrient 
      removal (MiDas-DK) - a tool for understanding activated sludge population 
      dynamics and community stability.
PG  - 2519-26
LID - 10.2166/wst.2013.151 [doi]
AB  - Since 2006 more than 50 Danish full-scale wastewater treatment plants with 
      nutrient removal have been investigated in a project called 'The Microbial 
      Database for Danish Activated Sludge Wastewater Treatment Plants with Nutrient 
      Removal (MiDas-DK)'. Comprehensive sets of samples have been collected, analyzed 
      and associated with extensive operational data from the plants. The community 
      composition was analyzed by quantitative fluorescence in situ hybridization 
      (FISH) supported by 16S rRNA amplicon sequencing and deep metagenomics. MiDas-DK 
      has been a powerful tool to study the complex activated sludge ecosystems, and, 
      besides many scientific articles on fundamental issues on mixed communities 
      encompassing nitrifiers, denitrifiers, bacteria involved in P-removal, 
      hydrolysis, fermentation, and foaming, the project has provided results that can 
      be used to optimize the operation of full-scale plants and carry out 
      trouble-shooting. A core microbial community has been defined comprising the 
      majority of microorganisms present in the plants. Time series have been 
      established, providing an overview of temporal variations in the different 
      plants. Interestingly, although most microorganisms were present in all plants, 
      there seemed to be plant-specific factors that controlled the population 
      composition thereby keeping it unique in each plant over time. Statistical 
      analyses of FISH and operational data revealed some correlations, but less than 
      expected. MiDas-DK (www.midasdk.dk) will continue over the next years and we hope 
      the approach can inspire others to make similar projects in other parts of the 
      world to get a more comprehensive understanding of microbial communities in 
      wastewater engineering.
FAU - Mielczarek, A T
AU  - Mielczarek AT
AD  - Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg 
      University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark.
FAU - Saunders, A M
AU  - Saunders AM
FAU - Larsen, P
AU  - Larsen P
FAU - Albertsen, M
AU  - Albertsen M
FAU - Stevenson, M
AU  - Stevenson M
FAU - Nielsen, J L
AU  - Nielsen JL
FAU - Nielsen, P H
AU  - Nielsen PH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Bacteria/genetics
MH  - *Databases, Factual
MH  - Denmark
MH  - In Situ Hybridization, Fluorescence
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - *Waste Disposal, Fluid
EDAT- 2013/06/12 06:00
MHDA- 2013/09/11 06:00
CRDT- 2013/06/12 06:00
PHST- 2013/06/12 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2013/09/11 06:00 [medline]
AID - 10.2166/wst.2013.151 [doi]
PST - ppublish
SO  - Water Sci Technol. 2013;67(11):2519-26. doi: 10.2166/wst.2013.151.