PMID- 23754667
OWN - NLM
STAT- MEDLINE
DCOM- 20140516
LR  - 20131004
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 57
IP  - 10
DP  - 2013 Oct
TI  - Methionine-induced hyperhomocysteinemia impairs the antioxidant ability of 
      high-density lipoproteins without reducing in vivo macrophage-specific reverse 
      cholesterol transport.
PG  - 1814-24
LID - 10.1002/mnfr.201300133 [doi]
AB  - SCOPE: High plasma homocysteine concentrations have been associated with 
      increased risk of cardiovascular disease both in humans and experimental animal 
      models, whereas plasma HDL-cholesterol concentration is inversely correlated with 
      such disorders. This work aimed to study the impact of methionine-induced 
      hyperhomocysteinemia (HHcy) on two major antiatherogenic functions of HDL, namely 
      their capacity to prevent LDL oxidation and induce in vivo macrophage-specific 
      reverse cholesterol transport. METHODS AND RESULTS: Methionine-induced HHcy in 
      mice resulted in an approximately 20% decreased concentration of HDL-cholesterol 
      and HDL main protein component, apolipoprotein A-I. The HDL potential to resist 
      oxidation as well as to prevent LDL oxidative modification was impaired in 
      hyperhomocysteinemic mice. Activities of paraoxonase-1 and platelet activation 
      factor acetylhydrolase, two of the main HDL-associated enzymes with antioxidant 
      activity, were reduced. The ability of HDL to efflux cholesterol from macrophages 
      was decreased in hyperhomocysteinemic mice; however, the in vivo 
      macrophage-specific reverse cholesterol transport measured as the output of 
      labeled cholesterol into feces did not significantly differ between groups. 
      CONCLUSION: Our data indicate that the HDL from methionine-induced 
      hyperhomocysteinemic mice was more prone to oxidation and displayed lower 
      capacity to protect LDL against oxidative modification than that of control mice, 
      highlighting a mechanism by which a diet-induced HHcy may facilitate progression 
      of atherosclerosis.
CI  - (c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Julve, Josep
AU  - Julve J
AD  - Institut d'Investigacions Biomediques Sant Pau (IIB-Sant Pau), Barcelona, Spain; 
      CIBER de Diabetes y Enfermedades Metabolicas Asociadas, CIBERDEM, Spain.
FAU - Escola-Gil, Joan C
AU  - Escola-Gil JC
FAU - Rodriguez-Millan, Elisabeth
AU  - Rodriguez-Millan E
FAU - Martin-Campos, Jesus M
AU  - Martin-Campos JM
FAU - Jauhiainen, Matti
AU  - Jauhiainen M
FAU - Quesada, Helena
AU  - Quesada H
FAU - Renteria-Obregon, Ivy M
AU  - Renteria-Obregon IM
FAU - Osada, Jesus
AU  - Osada J
FAU - Sanchez-Quesada, Jose L
AU  - Sanchez-Quesada JL
FAU - Blanco-Vaca, Francisco
AU  - Blanco-Vaca F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130610
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Antioxidants)
RN  - 0 (Apolipoprotein A-I)
RN  - 0 (Cholesterol, HDL)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - AE28F7PNPL (Methionine)
RN  - EC 3.1.8.1 (Aryldialkylphosphatase)
RN  - Homocysteinemia
SB  - IM
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Apolipoprotein A-I/blood
MH  - Aryldialkylphosphatase/blood
MH  - Atherosclerosis/pathology
MH  - Biological Transport
MH  - Cell Line
MH  - Cholesterol, HDL/*antagonists & inhibitors/blood
MH  - Disease Models, Animal
MH  - Female
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/*chemically induced/complications/pathology
MH  - Lipid Metabolism/drug effects
MH  - Liver/drug effects/metabolism
MH  - Macrophages/*metabolism
MH  - Male
MH  - Methionine/administration & dosage/*adverse effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
OTO - NOTNLM
OT  - HDL
OT  - Homocysteine
OT  - Hyperhomocysteinemia
OT  - Methionine
OT  - Oxidation
OT  - Reverse cholesterol transport
EDAT- 2013/06/12 06:00
MHDA- 2014/05/17 06:00
CRDT- 2013/06/12 06:00
PHST- 2013/02/20 00:00 [received]
PHST- 2013/03/26 00:00 [revised]
PHST- 2013/03/31 00:00 [accepted]
PHST- 2013/06/12 06:00 [entrez]
PHST- 2013/06/12 06:00 [pubmed]
PHST- 2014/05/17 06:00 [medline]
AID - 10.1002/mnfr.201300133 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2013 Oct;57(10):1814-24. doi: 10.1002/mnfr.201300133. Epub 
      2013 Jun 10.