PMID- 23756150
OWN - NLM
STAT- MEDLINE
DCOM- 20140509
LR  - 20131021
IS  - 1873-4863 (Electronic)
IS  - 0168-1656 (Linking)
VI  - 168
IP  - 2
DP  - 2013 Oct 20
TI  - Magnetic field intensified bi-enzyme system with in situ cofactor regeneration 
      supported by magnetic nanoparticles.
PG  - 212-7
LID - S0168-1656(13)00248-4 [pii]
LID - 10.1016/j.jbiotec.2013.05.016 [doi]
AB  - Efficient dynamic interactions among cofactor, enzymes and substrate molecules 
      are of primary importance for multi-step enzymatic reactions with in situ 
      cofactor regeneration. Here we showed for the first time that the above dynamic 
      interactions could be significantly intensified by exerting an external 
      alternating magnetic field on magnetic nanoparticles-supported multi-enzymatic 
      system so that the inter-particle collisions due to Brownian motion of 
      nanoparticles could be improved. To that end, a multienzyme system including 
      glutamate dehydrogenase (GluDH), glucose dehydrogenase (GDH) and cofactor NAD(H) 
      were separately immobilized on silica coated Fe3O4 magnetic nanoparticles with an 
      average diameter of 105 nm, and the effect of magnetic field strength and 
      frequency on the kinetics of the coupled bi-enzyme reaction was investigated. It 
      was found that at low magnetic field frequency (25 Hz and 100 Hz), increasing 
      magnetic field strength from 9.8 to 161.1 Gs led to only very slight increase in 
      reaction rate of the coupled bi-enzyme reaction expressed by glucose consumption 
      rate. At higher magnetic field of 200 Hz and 500 Hz, reaction rate increased 
      significantly with increase of magnetic field strength. When the magnetic field 
      frequency was kept at 500 Hz, the reaction rate increased from 3.89 muM/min to 
      8.11 muM/min by increasing magnetic field strength from 1.3 to 14.2 Gs. The 
      immobilized bi-enzyme system also showed good reusability and stability in the 
      magnetic field (500 Hz, 14.2 Gs), that about 46% of original activity could be 
      retained after 33 repeated uses, accounting for totally 34 days continuous 
      operation. These results demonstrated the feasibility in intensifying molecular 
      interactions among magnetic nanoparticle-supported multienzymes by using 
      nano-magnetic stirrer for efficient multi-step transformations.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Zheng, Muqing
AU  - Zheng M
AD  - National Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Su, Zhiguo
AU  - Su Z
FAU - Ji, Xiaoyuan
AU  - Ji X
FAU - Ma, Guanghui
AU  - Ma G
FAU - Wang, Ping
AU  - Wang P
FAU - Zhang, Songping
AU  - Zhang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130610
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (Coenzymes)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Multienzyme Complexes)
RN  - 0U46U6E8UK (NAD)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 1.1.1.47 (Glucose 1-Dehydrogenase)
RN  - EC 1.4.1.2 (Glutamate Dehydrogenase)
SB  - IM
MH  - Coenzymes/*metabolism
MH  - Enzyme Stability
MH  - Enzymes, Immobilized/chemistry/metabolism
MH  - Glucose 1-Dehydrogenase/chemistry/*metabolism
MH  - Glutamate Dehydrogenase/*metabolism
MH  - Kinetics
MH  - *Magnetic Fields
MH  - Multienzyme Complexes/metabolism
MH  - NAD/chemistry/*metabolism
MH  - Nanoparticles/*chemistry
MH  - Silicon Dioxide/chemistry
OTO - NOTNLM
OT  - Cofactor regeneration
OT  - Immobilization
OT  - Magnetic field
OT  - Magnetic nanoparticles
OT  - Multienzyme
EDAT- 2013/06/13 06:00
MHDA- 2014/05/10 06:00
CRDT- 2013/06/13 06:00
PHST- 2012/12/15 00:00 [received]
PHST- 2013/05/29 00:00 [revised]
PHST- 2013/05/30 00:00 [accepted]
PHST- 2013/06/13 06:00 [entrez]
PHST- 2013/06/13 06:00 [pubmed]
PHST- 2014/05/10 06:00 [medline]
AID - S0168-1656(13)00248-4 [pii]
AID - 10.1016/j.jbiotec.2013.05.016 [doi]
PST - ppublish
SO  - J Biotechnol. 2013 Oct 20;168(2):212-7. doi: 10.1016/j.jbiotec.2013.05.016. Epub 
      2013 Jun 10.