PMID- 23756809
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20131121
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 436
IP  - 4
DP  - 2013 Jul 12
TI  - Far-infrared radiation acutely increases nitric oxide production by increasing 
      Ca(2+) mobilization and Ca(2+)/calmodulin-dependent protein kinase II-mediated 
      phosphorylation of endothelial nitric oxide synthase at serine 1179.
PG  - 601-6
LID - S0006-291X(13)00956-X [pii]
LID - 10.1016/j.bbrc.2013.06.003 [doi]
AB  - Repeated thermal therapy manifested by far-infrared (FIR) radiation improves 
      vascular function in both patients and mouse model with coronary heart disease, 
      but its underlying mechanism is not fully understood. Using FIR as a thermal 
      therapy agent, we investigate the molecular mechanism of its effect on 
      endothelial nitric oxide synthase (eNOS) activity and NO production. FIR 
      increased the phosphorylation of eNOS at serine 1179 (eNOS-Ser(1179)) in a 
      time-dependent manner (up to 40min of FIR radiation) in bovine aortic endothelial 
      cells (BAEC) without alterations in eNOS expression. This increase was 
      accompanied by increases in NO production and intracellular Ca(2+) levels. 
      Treatment with KN-93, a selective inhibitor of Ca(2+)/calmodulin-dependent 
      protein kinase II (CaMKII) and H-89, a protein kinase A inhibitor, inhibited FIR 
      radiation-stimulated eNOS-Ser(1179) phosphorylation. FIR radiation itself also 
      increased the temperature of culture medium. As transient receptors potential 
      vanilloid (TRPV) ion channels are known to be temperature-sensitive calcium 
      channels, we explore whether TRPV channels mediate these observed effects. 
      Reverse transcription-PCR assay revealed two TRPV isoforms in BAEC, TRPV2 and 
      TRPV4. Although ruthenium red, a pan-TRPV inhibitor, completely reversed the 
      observed effect of FIR radiation, a partial attenuation ( approximately 20%) was found in cells 
      treated with Tranilast, TRPV2 inhibitor. However, ectopic expression of siRNA of 
      TRPV2 showed no significant alteration in FIR radiation-stimulated eNOS-Ser(1179) 
      phosphorylation. This study suggests that FIR radiation increases NO production 
      via increasing CaMKII-mediated eNOS-Ser(1179) phosphorylation but TRPV channels 
      may not be involved in this pathway. Our results may provide the molecular 
      mechanism by which FIR radiation improves endothelial function.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Park, Jung-Hyun
AU  - Park JH
AD  - Department of Molecular Medicine and Ewha Medical Research Institute, Ewha Womans 
      University Medical School, Seoul 158-710, South Korea.
FAU - Lee, Sangmi
AU  - Lee S
FAU - Cho, Du-Hyong
AU  - Cho DH
FAU - Park, Young Mi
AU  - Park YM
FAU - Kang, Duk-Hee
AU  - Kang DH
FAU - Jo, Inho
AU  - Jo I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130610
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 452VLY9402 (Serine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcium/*metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/*metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - DNA Primers
MH  - *Infrared Rays
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase Type III/chemistry/*metabolism
MH  - Phosphorylation
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serine/*metabolism
OTO - NOTNLM
OT  - AMP-activated protein kinase
OT  - AMPK
OT  - BAEC
OT  - Ca(2+)/calmodulin-dependent protein kinase II
OT  - CaMKII
OT  - EC
OT  - Endothelial nitric oxide synthase
OT  - FIR
OT  - Far-infrared
OT  - HUVEC
OT  - NO
OT  - Nitric oxide
OT  - PKA
OT  - Phosphorylation
OT  - TRP
OT  - TRP ankyrin
OT  - TRP melastatin
OT  - TRP vanilloid
OT  - TRPA
OT  - TRPM
OT  - TRPV
OT  - Transient receptor potential vanilloid ion channel
OT  - bovine aortic EC
OT  - eNOS
OT  - eNOS at serine 1179
OT  - eNOS-Ser(1179)
OT  - endothelial cell(s)
OT  - endothelial nitric oxide synthase
OT  - far-infrared
OT  - human umbilical vein EC
OT  - nitric oxide
OT  - protein kinase A
OT  - siRNA
OT  - small interference RNA
OT  - transient receptors potential
EDAT- 2013/06/13 06:00
MHDA- 2013/10/22 06:00
CRDT- 2013/06/13 06:00
PHST- 2013/05/28 00:00 [received]
PHST- 2013/06/01 00:00 [accepted]
PHST- 2013/06/13 06:00 [entrez]
PHST- 2013/06/13 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
AID - S0006-291X(13)00956-X [pii]
AID - 10.1016/j.bbrc.2013.06.003 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2013 Jul 12;436(4):601-6. doi: 
      10.1016/j.bbrc.2013.06.003. Epub 2013 Jun 10.