PMID- 23756945
OWN - NLM
STAT- MEDLINE
DCOM- 20140328
LR  - 20130612
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1029
DP  - 2013
TI  - Mouse ES cell-derived hematopoietic progenitor cells.
PG  - 109-17
LID - 10.1007/978-1-62703-478-4_8 [doi]
AB  - Future stem cell-based therapies will benefit from the new discoveries being made 
      on pluripotent stem cells such as embryonic stem (ES) cells and induced 
      pluripotent stem (IPS) cells. Understanding the genes regulating pluripotency has 
      opened new opportunities to generate patient-tailored therapies. However, 
      protocols for deriving progenitor cells of therapeutic grade from these 
      pluripotent stem cells are not yet worked out. In particular the potential of 
      these cells in treating diseases when compared to their adult progenitor 
      counterparts is unknown. This is crucial work that needs to be studied in detail 
      because we will need to determine engraftment potential of these cells and their 
      ability for multi-lineage engraftment in the in vivo setting before any clinical 
      applications. The ability of these cells to engraft is dependent on their 
      expression of cell surface markers which guide their homing patterns. In this 
      review, I discuss murine hematopoietic progenitor cells derived from mouse ES 
      cells. Stem cells in the bone marrow are found in the bone marrow niches. Our 
      knowledge of the bone marrow niches is growing and will ultimately lead to 
      improved clinical transplantation of bone marrow cells. We are, however, a long 
      way in appreciating how hematopoietic progenitor cells migrate and populate 
      lymphoid tissues. One of the variables in generating hematopoietic progenitor 
      cells is that different labs use different approaches in generating progenitor 
      cells. In some cases, the ES cell lines used show some variability as well. The 
      cell culture media used by the different investigators highly influence the 
      maturation level of the cells and their homing patterns. Here, mouse ES 
      cell-derived progenitor cells are discussed.
FAU - Kim, Eun-Mi
AU  - Kim EM
AD  - Department of Internal Medicine, Division of Immunology, University of Iowa, Iowa 
      City, IA, USA.
FAU - Manzar, Gohar
AU  - Manzar G
FAU - Zavazava, Nicholas
AU  - Zavazava N
LA  - eng
GR  - 3 R01 HL073015-04A1S1/HL/NHLBI NIH HHS/United States
GR  - 5R01 HL073015-08/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Cell Lineage
MH  - Cell Survival
MH  - Embryonic Stem Cells/*cytology/metabolism
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Induced Pluripotent Stem Cells/cytology/metabolism
MH  - Mice
EDAT- 2013/06/13 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/06/13 06:00
PHST- 2013/06/13 06:00 [entrez]
PHST- 2013/06/13 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - 10.1007/978-1-62703-478-4_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;1029:109-17. doi: 10.1007/978-1-62703-478-4_8.