PMID- 23757227 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20130614 LR - 20211021 IS - 1758-2229 (Electronic) IS - 1758-2229 (Linking) VI - 4 IP - 1 DP - 2012 Feb TI - Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities. PG - 36-41 LID - 10.1111/j.1758-2229.2011.00275.x [doi] AB - Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems. CI - (c) 2011 Society for Applied Microbiology and Blackwell Publishing Ltd. FAU - Knierim, Bernhard AU - Knierim B AD - Lawrence Berkeley National Laboratory, Life Sciences Division, Berkeley, CA, USA. Department of Earth and Planetary Science Department of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, CA, USA. Centre for Microscopy and Microanalysis (CMM), The University of Queensland, St. Lucia, Queensland, Australia. FAU - Luef, Birgit AU - Luef B FAU - Wilmes, Paul AU - Wilmes P FAU - Webb, Richard I AU - Webb RI FAU - Auer, Manfred AU - Auer M FAU - Comolli, Luis R AU - Comolli LR FAU - Banfield, Jillian F AU - Banfield JF LA - eng GR - P01 GM051487/GM/NIGMS NIH HHS/United States PT - Journal Article DEP - 20110822 PL - United States TA - Environ Microbiol Rep JT - Environmental microbiology reports JID - 101499207 PMC - PMC4444221 MID - NIHMS629306 EDAT- 2012/02/01 00:00 MHDA- 2012/02/01 00:01 PMCR- 2015/05/26 CRDT- 2013/06/13 06:00 PHST- 2013/06/13 06:00 [entrez] PHST- 2012/02/01 00:00 [pubmed] PHST- 2012/02/01 00:01 [medline] PHST- 2015/05/26 00:00 [pmc-release] AID - 10.1111/j.1758-2229.2011.00275.x [doi] PST - ppublish SO - Environ Microbiol Rep. 2012 Feb;4(1):36-41. doi: 10.1111/j.1758-2229.2011.00275.x. Epub 2011 Aug 22.