PMID- 23758346
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20130716
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 85
IP  - 14
DP  - 2013 Jul 16
TI  - In situ live cell sensing of multiple nucleotides exploiting DNA/RNA aptamers and 
      graphene oxide nanosheets.
PG  - 6775-82
LID - 10.1021/ac400858g [doi]
AB  - Nucleotides, for example, adenosine-5'-triphosphate (ATP) and 
      guanosine-5'-triphosphate (GTP), are primary energy resources for numerous 
      reactions in organisms including microtubule assembly, insulin secretion, ion 
      channel regulation, and so on. In order to advance our understanding of the 
      production and consumption of nucleoside triphosphates, a versatile sensing 
      platform for simultaneous visualization of ATP, GTP, adenosine derivates, and 
      guanosine derivates in living cells has been built up in the present work based 
      on graphene oxide nanosheets (GO-nS) and DNA/RNA aptamers. Taking advantage of 
      the robust fluorescence quenching ability, unique adsorption for single-strand 
      DNA/RNA probes, and efficient intracellular transport capacity of GO-nS, 
      selective and sensitive visualization of multiple nucleoside triphosphates in 
      living cells is successfully realized with the designed aptamer/GO-nS sensing 
      platform. Moreover, GO-nS displays good biocompatibility to living cells and high 
      protecting ability for DNA/RNA probes from enzymatic cleavage. These results 
      demonstrate that the aptamers/GO-nS-based sensing platform is capable of 
      selective, simultaneous, and in situ detection of multiple nucleotides, which 
      hold a great potential for analyzing other biomolecules in living cells.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and 
      Instrumentation, Tsinghua University, Beijing, China.
FAU - Li, Zhaohui
AU  - Li Z
FAU - Weber, Thomas J
AU  - Weber TJ
FAU - Hu, Dehong
AU  - Hu D
FAU - Lin, Chiann-Tso
AU  - Lin CT
FAU - Li, Jinghong
AU  - Li J
FAU - Lin, Yuehe
AU  - Lin Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130624
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Nucleotides)
RN  - 0 (Oxides)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Aptamers, Nucleotide/*analysis
MH  - Biosensing Techniques/*methods
MH  - Graphite/*chemistry
MH  - Humans
MH  - MCF-7 Cells
MH  - Nanostructures/*chemistry
MH  - Nucleotides/analysis
MH  - Oxides/chemistry
MH  - X-Ray Diffraction
EDAT- 2013/06/14 06:00
MHDA- 2014/04/23 06:00
CRDT- 2013/06/14 06:00
PHST- 2013/06/14 06:00 [entrez]
PHST- 2013/06/14 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
AID - 10.1021/ac400858g [doi]
PST - ppublish
SO  - Anal Chem. 2013 Jul 16;85(14):6775-82. doi: 10.1021/ac400858g. Epub 2013 Jun 24.