PMID- 23761663
OWN - NLM
STAT- MEDLINE
DCOM- 20140213
LR  - 20211021
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 20
IP  - 8
DP  - 2013 Aug
TI  - Identification of Haemophilus influenzae clones associated with invasive disease 
      a decade after introduction of H. influenzae serotype b vaccination in Italy.
PG  - 1223-9
LID - 10.1128/CVI.00028-13 [doi]
AB  - The introduction of Haemophilus influenzae serotype b (Hib) conjugate vaccines 
      has changed the epidemiology of invasive H. influenzae disease, with a shift in 
      the predominant serotype from Hib to nonencapsulated H. influenzae (ncHi). The 
      objective of this study was to identify the genotypes/clones associated with 
      invasive H. influenzae disease in Italy. Eighty-seven H. influenzae strains 
      isolated in the years 2009 to 2011 within the National Surveillance of Invasive 
      Bacterial Disease program were analyzed. Strains were characterized by 
      serotyping, antimicrobial susceptibility testing, and multilocus sequence typing 
      (MLST). Genetic polymorphisms in the bla(TEM) gene promoter region as well as the 
      occurrence of both adhesin genes (hmwA and hia) and the IgA1 protease-encoding 
      gene (igaB) were also investigated. Of 87 strains, 67 were ncHi and 20 were 
      encapsulated. Eleven strains were beta-lactamase positive, harboring the bla(TEM) 
      gene. Most bla(TEM) genes (10/11) were associated with a Pdel promoter region 
      exhibiting a 135-bp deletion; the remaining strain possessed the Pa/Pb 
      overlapping promoter. MLST analysis showed that encapsulated isolates were 
      clonal, with each serotype sharing a few related sequence types (STs). Forty-six 
      different STs were identified among the 67 ncHi strains. Despite this 
      heterogeneity, a group of closely related STs (ST103, ST139, and ST145) 
      encompassed almost 25% of all ncHi strains and 45.5% of the beta-lactamase producers 
      carrying the Pdel promoter. These major ST clones were found to be associated 
      with the hmwA gene but not with the igaB gene. To conclude, although the 
      heterogeneity of the ncHi population was confirmed, diffusion of major successful 
      ST clones was documented.
FAU - Giufre, Maria
AU  - Giufre M
AD  - Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto 
      Superiore di Sanita, Rome, Italy. marina.cerquetti@iss.it
FAU - Cardines, Rita
AU  - Cardines R
FAU - Accogli, Marisa
AU  - Accogli M
FAU - Pardini, Manuela
AU  - Pardini M
FAU - Cerquetti, Marina
AU  - Cerquetti M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130612
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Haemophilus Vaccines)
RN  - 0 (Haemophilus influenzae type b polysaccharide vaccine)
RN  - 0 (Vaccines, Conjugate)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Bacteremia/*epidemiology/*microbiology
MH  - Bacterial Capsules/immunology
MH  - Genes, Bacterial
MH  - Genotype
MH  - Haemophilus Infections/*epidemiology/*microbiology
MH  - Haemophilus Vaccines/administration & dosage/immunology
MH  - Haemophilus influenzae/*classification/genetics/isolation & purification
MH  - Humans
MH  - Italy/epidemiology
MH  - Meningitis, Bacterial/*epidemiology/*microbiology
MH  - Microbial Sensitivity Tests
MH  - Molecular Epidemiology
MH  - Multilocus Sequence Typing
MH  - Serotyping
MH  - Vaccination/methods
MH  - Vaccines, Conjugate/administration & dosage/immunology
MH  - beta-Lactamases/genetics
PMC - PMC3754497
EDAT- 2013/06/14 06:00
MHDA- 2014/02/14 06:00
PMCR- 2014/02/01
CRDT- 2013/06/14 06:00
PHST- 2013/06/14 06:00 [entrez]
PHST- 2013/06/14 06:00 [pubmed]
PHST- 2014/02/14 06:00 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - CVI.00028-13 [pii]
AID - 00028-13 [pii]
AID - 10.1128/CVI.00028-13 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2013 Aug;20(8):1223-9. doi: 10.1128/CVI.00028-13. Epub 2013 
      Jun 12.