PMID- 23762843
OWN - NLM
STAT- MEDLINE
DCOM- 20131204
LR  - 20211021
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2013
DP  - 2013
TI  - Characterization of dendritic cell and regulatory T cell functions against 
      Mycobacterium tuberculosis infection.
PG  - 402827
LID - 10.1155/2013/402827 [doi]
LID - 402827
AB  - Glutathione (GSH) is a tripeptide that regulates intracellular redox and other 
      vital aspects of cellular functions. GSH plays a major role in enhancing the 
      immune system. Dendritic cells (DCs) are potent antigen presenting cells that 
      participate in both innate and acquired immune responses against microbial 
      infections. Regulatory T cells (Tregs) play a significant role in immune 
      homeostasis. In this study, we investigated the effects of GSH in enhancing the 
      innate and adaptive immune functions of DCs against Mycobacterium tuberculosis 
      (M. tb) infection. We also characterized the functions of the sub-populations of 
      CD4+T cells such as Tregs and non-Tregs in modulating the ability of monocytes to 
      control the intracellular M. tb infection. Our results indicate that GSH by its 
      direct antimycobacterial activity inhibits the growth of intracellular M. tb 
      inside DCs. GSH also increases the expressions of costimulatory molecules such as 
      HLA-DR, CD80 and CD86 on the cell surface of DCs. Furthermore, GSH-enhanced DCs 
      induced a higher level of T-cell proliferation. We also observed that enhancing 
      the levels of GSH in Tregs resulted in downregulation in the levels of IL-10 and 
      TGF- beta and reduction in the fold growth of M. tb inside monocytes. Our studies 
      demonstrate novel regulatory mechanisms that favor both innate and adaptive 
      control of M. tb infection.
FAU - Morris, Devin
AU  - Morris D
AD  - Department of Basic Medical Sciences, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 East Second Street, Pomona, 
      CA 91766-1854, USA.
FAU - Gonzalez, Brenda
AU  - Gonzalez B
FAU - Khurasany, Melissa
AU  - Khurasany M
FAU - Kassissa, Christine
AU  - Kassissa C
FAU - Luong, Jennifer
AU  - Luong J
FAU - Kasko, Sarah
AU  - Kasko S
FAU - Pandya, Shalin
AU  - Pandya S
FAU - Chu, Michael
AU  - Chu M
FAU - Chi, Po-Ting
AU  - Chi PT
FAU - Bui, Steven
AU  - Bui S
FAU - Guerra, Carlos
AU  - Guerra C
FAU - Chan, John
AU  - Chan J
FAU - Venketaraman, Vishwanath
AU  - Venketaraman V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130523
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Biomarkers)
RN  - 0 (Cell Extracts)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Analysis of Variance
MH  - Biomarkers/metabolism
MH  - Cell Extracts
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Dendritic Cells/drug effects/*immunology/microbiology
MH  - Glutathione/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Intracellular Space/microbiology
MH  - Lipopolysaccharides/pharmacology
MH  - Microbial Viability/drug effects
MH  - Mycobacterium tuberculosis/drug effects/*immunology
MH  - T-Lymphocytes, Regulatory/cytology/drug effects/*immunology
MH  - Tuberculosis/*immunology/*microbiology
PMC - PMC3676983
EDAT- 2013/06/14 06:00
MHDA- 2013/12/16 06:00
PMCR- 2013/05/23
CRDT- 2013/06/14 06:00
PHST- 2013/03/25 00:00 [received]
PHST- 2013/04/28 00:00 [revised]
PHST- 2013/05/08 00:00 [accepted]
PHST- 2013/06/14 06:00 [entrez]
PHST- 2013/06/14 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2013/05/23 00:00 [pmc-release]
AID - 10.1155/2013/402827 [doi]
PST - ppublish
SO  - Biomed Res Int. 2013;2013:402827. doi: 10.1155/2013/402827. Epub 2013 May 23.