PMID- 23763865
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20161125
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 93
IP  - 1
DP  - 2013 Sep
TI  - Disruption of biomolecule function by nanoparticles: how do gold nanoparticles 
      affect Phase I biotransformation of persistent organic pollutants?
PG  - 123-32
LID - S0045-6535(13)00723-6 [pii]
LID - 10.1016/j.chemosphere.2013.05.004 [doi]
AB  - The potential influence of nanoparticles on cytochrome P-450 (CYP) isozyme 
      mediated Phase I biotransformation of persistent organic pollutants (POPs) in 
      vitro was investigated using citrate-capped gold nanoparticles (AuNPs) and 
      2,2',3,5',6-pentachlorobiphenyl (PCB 95) as the probe nanoparticle and compound, 
      respectively. AuNPs affected the biotransformation activity of rat CYP2B1 and 
      changed the atropisomeric composition of PCB 95, depending on the incubation time 
      and the AuNP concentration. Electrostatic repulsion between citrate-coated AuNPs 
      and rat CYP2B1 may influence the active conformation of the isozyme and 
      consequently affect its activity and stereoselectivity. In addition, the effects 
      of AuNPs on rat CYP2B1 activity also appeared to be through interference with the 
      CYP catalytic cycle's electron transfer chain. Incubations with AuNPs had a 
      decline in buffer conductance and an absorbance band red shift of AuNPs, from 
      electrostatic interactions of K(+) with negatively-charged AuNP aggregates. These 
      ionic strength changes affected the formation rate of nicotinamide adenine 
      dinucleotide phosphate, which provides electrons for the oxidative reaction 
      cycle, and the biotransformation activity and stereoselectivity of CYP. This 
      study suggests that charged nanoparticles may be able to alter the functions of 
      biomolecules directly, by electrostatic interaction, or indirectly, by changes to 
      the surrounding ionic strength. These factors should be taken into account for 
      further understanding and prediction of the environmental behavior and fate of 
      POPs and nanoparticles.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Lu, Zhe
AU  - Lu Z
AD  - Department of Chemistry, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.
FAU - Ma, Guibin
AU  - Ma G
FAU - Veinot, Jonathan G C
AU  - Veinot JG
FAU - Wong, Charles S
AU  - Wong CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130610
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Buffers)
RN  - 0 (Environmental Pollutants)
RN  - 0YO8J06WCR (2,2',3,5',6-pentachlorobiphenyl)
RN  - 2968PHW8QP (Citric Acid)
RN  - 53-59-8 (NADP)
RN  - 7440-57-5 (Gold)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP2B1)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Animals
MH  - Buffers
MH  - Citric Acid/chemistry
MH  - Cytochrome P-450 CYP2B1/metabolism
MH  - Environmental Pollutants/*metabolism
MH  - Gold/*chemistry/*pharmacology
MH  - *Metabolic Detoxication, Phase I
MH  - *Metal Nanoparticles
MH  - NADP/biosynthesis
MH  - Polychlorinated Biphenyls/*metabolism
MH  - Potassium/chemistry
MH  - Rats
MH  - Salinity
MH  - Stereoisomerism
OTO - NOTNLM
OT  - Chiral organic pollutants
OT  - Cytochrome P-450
OT  - Disruption
OT  - Ionic strength
OT  - Nanoparticles
EDAT- 2013/06/15 06:00
MHDA- 2014/03/07 06:00
CRDT- 2013/06/15 06:00
PHST- 2013/02/02 00:00 [received]
PHST- 2013/04/23 00:00 [revised]
PHST- 2013/05/02 00:00 [accepted]
PHST- 2013/06/15 06:00 [entrez]
PHST- 2013/06/15 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
AID - S0045-6535(13)00723-6 [pii]
AID - 10.1016/j.chemosphere.2013.05.004 [doi]
PST - ppublish
SO  - Chemosphere. 2013 Sep;93(1):123-32. doi: 10.1016/j.chemosphere.2013.05.004. Epub 
      2013 Jun 10.