PMID- 23766023
OWN - NLM
STAT- MEDLINE
DCOM- 20140501
LR  - 20181202
IS  - 1098-2396 (Electronic)
IS  - 0887-4476 (Linking)
VI  - 67
IP  - 11
DP  - 2013 Nov
TI  - Metabotropic glutamate receptor 2 and corticotrophin-releasing factor receptor-1 
      gene expression is differently regulated by BDNF in rat primary cortical neurons.
PG  - 794-800
LID - 10.1002/syn.21689 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is important for neuronal survival and 
      plasticity. Incorporation of matured receptor proteins is an integral part of 
      synapse formation. However, whether BDNF increases synthesis and integration of 
      receptors in functional synapses directly is unclear. We are particularly 
      interested in the regulation of the 5-hydroxytryptamine receptor 2A (5-HT(2A)R). 
      This receptor form a functional complex with the metabotropic glutamate receptor 
      2 (mGluR2) and is recruited to the cell membrane by the corticotrophin-releasing 
      factor receptor 1 (CRF-R1). The effect of BDNF on gene expression for all these 
      receptors, as well as a number of immediate-early genes, was pharmacologically 
      characterized in primary neurons from rat frontal cortex. BDNF increased CRF-R1 
      mRNA levels up to fivefold, whereas mGluR2 mRNA levels were proportionally 
      downregulated. No effect on 5-HT(2A)R mRNA was seen. The effects were 
      dose-dependent with half-maximal effective concentrations (EC(50)) around 1 
      ng/ml. After 24 h of incubation with BDNF, CRF-R1 mRNA levels had returned to 
      baseline levels, whereas mGluR2 mRNA levels remained low. A significant reduction 
      of all three receptor transcripts was observed after neuronal depolarization 
      produced by high potassium. This study emphasizes the role of BDNF as an 
      important regulator of receptor compositions in the synapse and provides further 
      evidence that BDNF directly regulates important drug targets involved in 
      cognition and mood.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Jorgensen, Christinna V
AU  - Jorgensen CV
AD  - Neurobiology Research Unit, University Hospital Rigshospitalet, Copenhagen, 
      Denmark.
FAU - Klein, Anders B
AU  - Klein AB
FAU - El-Sayed, Mona
AU  - El-Sayed M
FAU - Knudsen, Gitte M
AU  - Knudsen GM
FAU - Mikkelsen, Jens D
AU  - Mikkelsen JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130724
PL  - United States
TA  - Synapse
JT  - Synapse (New York, N.Y.)
JID - 8806914
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Serotonin, 5-HT2A)
RN  - 0 (Receptors, Corticotropin-Releasing Hormone)
RN  - 0 (Receptors, Metabotropic Glutamate)
RN  - 0 (metabotropic glutamate receptor 2)
RN  - 5CLY6W2H1M (CRF receptor type 1)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Frontal Lobe/*metabolism
MH  - *Gene Expression Regulation
MH  - Genes, Immediate-Early
MH  - Membrane Potentials
MH  - Neurons/drug effects/*metabolism/physiology
MH  - Potassium/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Serotonin, 5-HT2A/genetics/metabolism
MH  - Receptors, Corticotropin-Releasing Hormone/genetics/*metabolism
MH  - Receptors, Metabotropic Glutamate/genetics/*metabolism
MH  - Synapses/metabolism
MH  - Transcription, Genetic
OTO - NOTNLM
OT  - corticotrophin-releasing factor receptor-1 (CRF-R1)
OT  - metabotropic glutamate receptor 2 (mGluR2)
OT  - receptor regulation
OT  - serotonin receptor 2A (5-HT2AR)
OT  - synapse
EDAT- 2013/06/15 06:00
MHDA- 2014/05/03 06:00
CRDT- 2013/06/15 06:00
PHST- 2013/02/18 00:00 [received]
PHST- 2013/06/03 00:00 [accepted]
PHST- 2013/06/15 06:00 [entrez]
PHST- 2013/06/15 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - 10.1002/syn.21689 [doi]
PST - ppublish
SO  - Synapse. 2013 Nov;67(11):794-800. doi: 10.1002/syn.21689. Epub 2013 Jul 24.