PMID- 23766365
OWN - NLM
STAT- MEDLINE
DCOM- 20140407
LR  - 20211021
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 162
IP  - 4
DP  - 2013 Aug
TI  - HAPLESS13, the Arabidopsis mu1 adaptin, is essential for protein sorting at the 
      trans-Golgi network/early endosome.
PG  - 1897-910
LID - 10.1104/pp.113.221051 [doi]
AB  - In plant cells, secretory and endocytic routes intersect at the trans-Golgi 
      network (TGN)/early endosome (EE), where cargos are further sorted correctly and 
      in a timely manner. Cargo sorting is essential for plant survival and therefore 
      necessitates complex molecular machinery. Adaptor proteins (APs) play key roles 
      in this process by recruiting coat proteins and selecting cargos for different 
      vesicle carriers. The micro1 subunit of AP-1 in Arabidopsis (Arabidopsis thaliana) 
      was recently identified at the TGN/EE and shown to be essential for cytokinesis. 
      However, little was known about other cellular activities affected by mutations 
      in AP-1 or the developmental consequences of such mutations. We report here that 
      HAPLESS13 (HAP13), the Arabidopsis micro1 adaptin, is essential for protein sorting 
      at the TGN/EE. Functional loss of HAP13 displayed pleiotropic developmental 
      defects, some of which were suggestive of disrupted auxin signaling. Consistent 
      with this, the asymmetric localization of PIN-FORMED2 (PIN2), an auxin 
      transporter, was compromised in the mutant. In addition, cell morphogenesis was 
      disrupted. We further demonstrate that HAP13 is critical for brefeldin 
      A-sensitive but wortmannin-insensitive post-Golgi trafficking. Our results show 
      that HAP13 is a key link in the sophisticated trafficking network in plant cells.
FAU - Wang, Jia-Gang
AU  - Wang JG
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Tai'an 271018, China.
FAU - Li, Sha
AU  - Li S
FAU - Zhao, Xin-Ying
AU  - Zhao XY
FAU - Zhou, Liang-Zi
AU  - Zhou LZ
FAU - Huang, Guo-Qiang
AU  - Huang GQ
FAU - Feng, Chong
AU  - Feng C
FAU - Zhang, Yan
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130613
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (AP1M2 protein, Arabidopsis)
RN  - 0 (Adaptor Protein Complex 1)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Indoleacetic Acids)
RN  - 20350-15-6 (Brefeldin A)
SB  - IM
MH  - Adaptor Protein Complex 1/genetics/*metabolism
MH  - Arabidopsis/drug effects/genetics/growth & development/*metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Brefeldin A/pharmacology
MH  - Endosomes/*metabolism
MH  - Gene Expression Regulation, Plant
MH  - Indoleacetic Acids/metabolism
MH  - Mutation
MH  - Plants, Genetically Modified
MH  - *Protein Transport
MH  - Signal Transduction/genetics
MH  - trans-Golgi Network/*metabolism
PMC - PMC3729769
EDAT- 2013/06/15 06:00
MHDA- 2014/04/08 06:00
PMCR- 2013/06/13
CRDT- 2013/06/15 06:00
PHST- 2013/06/15 06:00 [entrez]
PHST- 2013/06/15 06:00 [pubmed]
PHST- 2014/04/08 06:00 [medline]
PHST- 2013/06/13 00:00 [pmc-release]
AID - pp.113.221051 [pii]
AID - 221051 [pii]
AID - 10.1104/pp.113.221051 [doi]
PST - ppublish
SO  - Plant Physiol. 2013 Aug;162(4):1897-910. doi: 10.1104/pp.113.221051. Epub 2013 
      Jun 13.