PMID- 23766563
OWN - NLM
STAT- MEDLINE
DCOM- 20131203
LR  - 20211021
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2013
DP  - 2013
TI  - High-mobility group box-1 induces decreased brain-derived neurotrophic 
      factor-mediated neuroprotection in the diabetic retina.
PG  - 863036
LID - 10.1155/2013/863036 [doi]
LID - 863036
AB  - To test the hypothesis that brain-derived neurotrophic factor-(BDNF-) mediated 
      neuroprotection is reduced by high-mobility group box-1 (HMGB1) in diabetic 
      retina, paired vitreous and serum samples from 46 proliferative diabetic 
      retinopathy and 34 nondiabetic patients were assayed for BDNF, HMGB1, soluble 
      receptor for advanced glycation end products (sRAGE), soluble intercellular 
      adhesion molecule-1 (sICAM-1), monocyte chemoattractant protein-1 (MCP-1), and 
      TBARS. We also examined retinas of diabetic and HMGB1 intravitreally injected 
      rats. The effect of the HMGB1 inhibitor glycyrrhizin on diabetes-induced changes 
      in retinal BDNF expressions was studied. Western blot, ELISA, and TBARS assays 
      were used. BDNF was not detected in vitreous samples. BDNF levels were 
      significantly lower in serum samples from diabetic patients compared with 
      nondiabetics, whereas HMGB1, sRAGE, sICAM-1, and TBARS levels were significantly 
      higher in diabetic serum samples. MCP-1 levels did not differ significantly. 
      There was significant inverse correlation between serum levels of BDNF and HMGB1. 
      Diabetes and intravitreal administration of HMGB1 induced significant 
      upregulation of the expression of HMGB1, TBARS, and cleaved caspase-3, whereas 
      the expression of BDNF and synaptophysin was significantly downregulated in rat 
      retinas. Glycyrrhizin significantly attenuated diabetes-induced downregulation of 
      BDNF. Our results suggest that HMGB1-induced downregulation of BDNF might be 
      involved in pathogenesis of diabetic retinal neurodegeneration.
FAU - Abu El-Asrar, Ahmed M
AU  - Abu El-Asrar AM
AD  - Department of Ophthalmology, College of Medicine, King Saud University, King 
      Abdulaziz University Hospital, Old Airport Road, PO Box 245, Riyadh 11411, Saudi 
      Arabia. abuasrar@ksu.edu.sa
FAU - Nawaz, Mohd Imtiaz
AU  - Nawaz MI
FAU - Siddiquei, Mohammad Mairaj
AU  - Siddiquei MM
FAU - Al-Kharashi, Abdullah S
AU  - Al-Kharashi AS
FAU - Kangave, Dustan
AU  - Kangave D
FAU - Mohammad, Ghulam
AU  - Mohammad G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130520
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chemokine CCL2)
RN  - 0 (HMGB1 Protein)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 6FO62043WK (Glycyrrhizic Acid)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*blood
MH  - Chemokine CCL2/metabolism
MH  - Diabetic Retinopathy/*blood/drug therapy
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Glycyrrhizic Acid/therapeutic use
MH  - HMGB1 Protein/*pharmacology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Male
MH  - Middle Aged
MH  - Oxidative Stress/drug effects
MH  - Rats, Sprague-Dawley
MH  - Young Adult
PMC - PMC3671668
EDAT- 2013/06/15 06:00
MHDA- 2013/12/16 06:00
PMCR- 2013/05/20
CRDT- 2013/06/15 06:00
PHST- 2013/04/15 00:00 [received]
PHST- 2013/04/25 00:00 [accepted]
PHST- 2013/06/15 06:00 [entrez]
PHST- 2013/06/15 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2013/05/20 00:00 [pmc-release]
AID - 10.1155/2013/863036 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2013;2013:863036. doi: 10.1155/2013/863036. Epub 2013 May 20.