PMID- 23767885
OWN - NLM
STAT- MEDLINE
DCOM- 20140512
LR  - 20181202
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 22
IP  - 21
DP  - 2013 Nov 1
TI  - Mesenchymal stem cells induce an inflammatory response after intravenous 
      infusion.
PG  - 2825-35
LID - 10.1089/scd.2013.0193 [doi]
AB  - Mesenchymal stem cells (MSCs) have potent immunosuppressive effects in vitro and 
      are considered as a therapeutic option for autoimmune disease and organ 
      transplantation. While MSCs show beneficial effects on immune disease progression 
      and transplant survival in animal models, the immunomodulatory mechanisms 
      involved are largely unknown. In the present study, we show that intravenously 
      infused C57BL/6- green fluorescent protein (GFP) MSCs home to the lungs in 
      C57BL/6 recipient mice and induce an inflammatory response. This response was 
      characterized by increased mRNA expression of monocyte chemoattractant protein-1 
      (MCP1), IL1-beta, and TNF-alpha and an increase in macrophages in lung tissue 2 h after 
      MSC infusion. Simultaneously, serum levels of proinflammatory IL6, CXCL1, and 
      MCP1 protein increased, demonstrating systemic immune activation after MSC 
      infusion. In liver tissue, no C57BL/6-GFP MSCs were detected, but MCP1 and TNF-alpha 
      mRNA levels peaked 4 h after MSC infusion. The expression of the 
      anti-inflammatory cytokines TGF-beta, IL4, and IL10 was only marginally affected. 
      Nevertheless, 3 days after MSC infusion, animals developed a milder inflammatory 
      response to lipopolysaccharides. Our results suggest that the in vivo 
      immunomodulatory effects of MSCs originate from an inflammatory response that is 
      induced by the infusion of MSCs, which is followed by a phase of reduced immune 
      reactivity.
FAU - Hoogduijn, Martin J
AU  - Hoogduijn MJ
AD  - 1 Nephrology and Transplantation, Department of Internal Medicine, Erasmus 
      Medical Center , Rotterdam, The Netherlands .
FAU - Roemeling-van Rhijn, Marieke
AU  - Roemeling-van Rhijn M
FAU - Engela, Anja U
AU  - Engela AU
FAU - Korevaar, Sander S
AU  - Korevaar SS
FAU - Mensah, Fane K F
AU  - Mensah FK
FAU - Franquesa, Marcella
AU  - Franquesa M
FAU - de Bruin, Ron W F
AU  - de Bruin RW
FAU - Betjes, Michiel G H
AU  - Betjes MG
FAU - Weimar, Willem
AU  - Weimar W
FAU - Baan, Carla C
AU  - Baan CC
LA  - eng
PT  - Journal Article
DEP - 20130809
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chemokines/blood/genetics/*immunology
MH  - Cytokines/blood/genetics/*immunology
MH  - Gene Expression/immunology
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Inflammation Mediators/blood/immunology/metabolism
MH  - Infusions, Intravenous
MH  - Kidney/immunology/metabolism
MH  - Lipopolysaccharides/immunology/pharmacology
MH  - Liver/immunology/metabolism
MH  - Lung/*immunology/metabolism
MH  - Macrophages/immunology/metabolism
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spleen/cytology/drug effects/immunology
MH  - Time Factors
EDAT- 2013/06/19 06:00
MHDA- 2014/05/13 06:00
CRDT- 2013/06/18 06:00
PHST- 2013/06/18 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/05/13 06:00 [medline]
AID - 10.1089/scd.2013.0193 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2013 Nov 1;22(21):2825-35. doi: 10.1089/scd.2013.0193. Epub 2013 
      Aug 9.