PMID- 23768324
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20130617
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 141
IP  - 1
DP  - 2013 Nov 1
TI  - Spectroscopic studies on the interaction of sodium benzoate, a food preservative, 
      with calf thymus DNA.
PG  - 41-7
LID - S0308-8146(13)00296-3 [pii]
LID - 10.1016/j.foodchem.2013.02.122 [doi]
AB  - The interaction between sodium benzoate (SB) and calf thymus DNA in simulated 
      physiological buffer (pH 7.4) using acridine orange (AO) dye as a fluorescence 
      probe, was investigated by UV-Vis absorption, fluorescence and circular dichroism 
      (CD) spectroscopy along with DNA melting studies and viscosity measurements. An 
      expanded UV-Vis spectral data matrix was resolved by multivariate curve 
      resolution-alternating least squares (MCR-ALS) approach. The equilibrium 
      concentration profiles and the pure spectra for SB, DNA and DNA-SB complex from 
      the high overlapping composite response were simultaneously obtained. The results 
      indicated that SB could bind to DNA, and hydrophobic interactions and hydrogen 
      bonds played a vital role in the binding process. Moreover, SB was able to quench 
      the fluorescence of DNA-AO complex through a static procedure. The quenching 
      observed was indicative of an intercalative mode of interaction between SB and 
      DNA, which was supported by melting studies, viscosity measurements and CD 
      analysis.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Zhang, Guowen
AU  - Zhang G
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 
      Nanjing East Road, Nanchang 330047, Jiangxi, China. gwzhang@ncu.edu.cn
FAU - Ma, Yadi
AU  - Ma Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130314
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Food Preservatives)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - F30N4O6XVV (Acridine Orange)
RN  - OJ245FE5EU (Sodium Benzoate)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cattle
MH  - Circular Dichroism
MH  - DNA/*chemistry
MH  - Fluorescent Dyes/chemistry
MH  - Food Preservatives/*chemistry
MH  - Kinetics
MH  - Sodium Benzoate/*chemistry
MH  - Spectrum Analysis
EDAT- 2013/06/19 06:00
MHDA- 2014/01/01 06:00
CRDT- 2013/06/18 06:00
PHST- 2012/10/16 00:00 [received]
PHST- 2013/01/16 00:00 [revised]
PHST- 2013/02/26 00:00 [accepted]
PHST- 2013/06/18 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - S0308-8146(13)00296-3 [pii]
AID - 10.1016/j.foodchem.2013.02.122 [doi]
PST - ppublish
SO  - Food Chem. 2013 Nov 1;141(1):41-7. doi: 10.1016/j.foodchem.2013.02.122. Epub 2013 
      Mar 14.