PMID- 23768360
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20130617
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 141
IP  - 1
DP  - 2013 Nov 1
TI  - The interaction of sesamol with DNA and cytotoxicity, apoptosis, and localization 
      in HepG2 cells.
PG  - 289-96
LID - S0308-8146(13)00268-9 [pii]
LID - 10.1016/j.foodchem.2013.02.105 [doi]
AB  - Sesamol, a nutritional antioxidant phenolic compound present in sesame seed, has 
      a potential therapeutic molecule effect against cancers. In this study, the 
      interaction between sesamol and DNA was investigated by employing 
      ultraviolet/visible (UV/Vis), fluorescence, circular dichroism (CD), Fourier 
      transform infrared spectroscopy (FT-IR), and molecular modeling. The fluorescence 
      analysis indicated that the fluorescence quenching mechanism of sesamol by calf 
      thymus DNA (ctDNA) occurred through static quenching. The UV/Vis, CD, FT-IR 
      spectra and molecular docking results implied that the primary binding mode was 
      minor groove binding. Furthermore, the intracellular interaction of sesamol with 
      DNA and its bioactivity effect were explored. The cell activity results 
      demonstrated that sesamol induced hepatic cell line (HepG2) death. The acridine 
      orange (AO)/ethidium bromide (EB) staining assay and DNA fragmentation confirmed 
      that sesamol could efficiently induce the apoptosis of HepG2 cells. Moreover, 
      addition of sesamol to HepG2 cells resulted in nuclear localization, as 
      visualized by confocal microscopy.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Liu, Zhigang
AU  - Liu Z
AD  - College of Food Science and Engineering, Northwest A&F University, Yangling, 
      China.
FAU - Xiang, Qisen
AU  - Xiang Q
FAU - Du, Lihui
AU  - Du L
FAU - Song, Ge
AU  - Song G
FAU - Wang, Yutang
AU  - Wang Y
FAU - Liu, Xuebo
AU  - Liu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130307
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Benzodioxoles)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - 94IEA0NV89 (sesamol)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Benzodioxoles/*chemistry/metabolism/*toxicity
MH  - Cattle
MH  - Cell Nucleus/drug effects/genetics/*metabolism
MH  - DNA/*chemistry
MH  - DNA Fragmentation/drug effects
MH  - Hep G2 Cells
MH  - Humans
MH  - Phenols/*chemistry/metabolism/*toxicity
MH  - Plant Extracts/*chemistry/metabolism/*toxicity
EDAT- 2013/06/19 06:00
MHDA- 2014/01/01 06:00
CRDT- 2013/06/18 06:00
PHST- 2012/09/07 00:00 [received]
PHST- 2013/01/10 00:00 [revised]
PHST- 2013/02/26 00:00 [accepted]
PHST- 2013/06/18 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - S0308-8146(13)00268-9 [pii]
AID - 10.1016/j.foodchem.2013.02.105 [doi]
PST - ppublish
SO  - Food Chem. 2013 Nov 1;141(1):289-96. doi: 10.1016/j.foodchem.2013.02.105. Epub 
      2013 Mar 7.