PMID- 23769811
OWN - NLM
STAT- MEDLINE
DCOM- 20131112
LR  - 20230621
IS  - 1095-6859 (Electronic)
IS  - 0090-8258 (Print)
IS  - 0090-8258 (Linking)
VI  - 130
IP  - 3
DP  - 2013 Sep
TI  - Chromosomal gains measured in cytology samples from women with abnormal cervical 
      cancer screening results.
PG  - 595-600
LID - S0090-8258(13)00838-X [pii]
LID - 10.1016/j.ygyno.2013.06.005 [doi]
AB  - OBJECTIVE: Chromosomal gains at 3q26, 5p15 and 20q13 have been described in 
      cervical precancer and cancer. We evaluated a novel fluorescence in situ 
      hybridization (FISH) assay that detects gains at these three loci simultaneously 
      as a possible biomarker for detecting cervical precancer. METHODS: Chromosomal 
      copy numbers at 3q26, 5p15, 20q13 and the centromere of chromosome7 (cen7) in 
      liquid-based cytology specimens from 168 women enrolled in the Biopsy Study were 
      determined by FISH. The number of cells with >/= 3 or >/= 4 signals for a genomic 
      locus was enumerated and diagnostic test performance measures were calculated 
      using receiver operating characteristic (ROC) analyses. Sensitivity and 
      specificity values were determined for the detection of CIN2+ and/or HSIL. 
      RESULTS: The median number of cells with >/= 3 signals increased with the severity 
      of cervical lesion for each genomic locus (p-trend<0.02 for each locus). ROC 
      analysis for the number of cells with >/= 3 signals resulted in area under the 
      curve values of 0.70 (95% CI: 0.54-0.86), 0.67 (0.52-0.83), 0.67 (0.51-0.83) and 
      0.78 (0.64-0.92) for 3q26, 5p15, 20q13 and cen7, respectively, for the detection 
      of CIN2+ and/or HSIL. Positivity for gains at multiple loci resulted in only 
      slightly better test performance measures than those for the individual probes 
      for four distinct combinations of probes. CONCLUSIONS: Chromosomal gains at 3q26, 
      5p15, 20q13 and cen7 are associated with severity of cervical lesions. Further 
      studies are required to quantify risk stratification of FISH assays for cervical 
      cancer screening.
CI  - Published by Elsevier Inc.
FAU - Luhn, Patricia
AU  - Luhn P
AD  - Division of Epidemiology and Genetics, National Cancer Institute, Rockville, MD, 
      USA. luhnpa@mail.nih.gov
FAU - Houldsworth, Jane
AU  - Houldsworth J
FAU - Cahill, Lynnette
AU  - Cahill L
FAU - Schiffman, Mark
AU  - Schiffman M
FAU - Castle, Philip E
AU  - Castle PE
FAU - Zuna, Rosemary E
AU  - Zuna RE
FAU - Dunn, S Terence
AU  - Dunn ST
FAU - Gold, Michael A
AU  - Gold MA
FAU - Walker, Joan
AU  - Walker J
FAU - Wentzensen, Nicolas
AU  - Wentzensen N
LA  - eng
GR  - R44 CA139667/CA/NCI NIH HHS/United States
GR  - ZIA CP010124/ImNIH/Intramural NIH HHS/United States
GR  - ZIA CP010124-17/ImNIH/Intramural NIH HHS/United States
GR  - R44CA139667/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130613
PL  - United States
TA  - Gynecol Oncol
JT  - Gynecologic oncology
JID - 0365304
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adult
MH  - Area Under Curve
MH  - Biomarkers, Tumor/*genetics
MH  - Chromosomes, Human, Pair 20
MH  - Chromosomes, Human, Pair 3
MH  - Chromosomes, Human, Pair 5
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Polyploidy
MH  - Precancerous Conditions/*genetics
MH  - ROC Curve
MH  - Uterine Cervical Neoplasms/*genetics/pathology
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/*genetics
PMC - PMC3833871
MID - NIHMS502979
OTO - NOTNLM
OT  - 3q26
OT  - Cervical cancer
OT  - FISH
OT  - Genomic gains
OT  - HPV
EDAT- 2013/06/19 06:00
MHDA- 2013/11/13 06:00
PMCR- 2014/09/01
CRDT- 2013/06/18 06:00
PHST- 2013/02/22 00:00 [received]
PHST- 2013/06/03 00:00 [revised]
PHST- 2013/06/06 00:00 [accepted]
PHST- 2013/06/18 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2013/11/13 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - S0090-8258(13)00838-X [pii]
AID - 10.1016/j.ygyno.2013.06.005 [doi]
PST - ppublish
SO  - Gynecol Oncol. 2013 Sep;130(3):595-600. doi: 10.1016/j.ygyno.2013.06.005. Epub 
      2013 Jun 13.