PMID- 23770097
OWN - NLM
STAT- MEDLINE
DCOM- 20131022
LR  - 20130726
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 587
IP  - 15
DP  - 2013 Aug 2
TI  - Insulin receptor substrate-1 (IRS-1) forms a ribonucleoprotein complex associated 
      with polysomes.
PG  - 2319-24
LID - S0014-5793(13)00450-X [pii]
LID - 10.1016/j.febslet.2013.05.066 [doi]
AB  - Insulin receptor substrates (IRSs) are known to play important roles in mediating 
      intracellular insulin-like growth factors (IGFs)/insulin signaling. In this 
      study, we identified components of messenger ribonucleoprotein (mRNP) as 
      IRS-1-associated proteins. IRS-1 complex formation analysis revealed that IRS-1 
      is incorporated into the complexes of molecular mass more than 1000 kDa, which 
      were disrupted by treatment with RNase. Furthermore, oligo(dT) beads precipitated 
      IRS-1 from cell lysates, showing that the IRS-1 complexes contained messenger 
      RNA. Taken together with the data that IRS-1 was fractionated into the 
      polysome-containing high-density fractions, we concluded that IRS-1 forms the 
      novel complexes with mRNPs.
CI  - Copyright (c) 2013 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Ozoe, Atsufumi
AU  - Ozoe A
AD  - Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo 
      113-8657, Japan.
FAU - Sone, Meri
AU  - Sone M
FAU - Fukushima, Toshiaki
AU  - Fukushima T
FAU - Kataoka, Naoyuki
AU  - Kataoka N
FAU - Arai, Toshiya
AU  - Arai T
FAU - Chida, Kazuhiro
AU  - Chida K
FAU - Asano, Tomoichiro
AU  - Asano T
FAU - Hakuno, Fumihiko
AU  - Hakuno F
FAU - Takahashi, Shin-Ichiro
AU  - Takahashi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130613
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Ribonucleoproteins)
SB  - IM
MH  - Cell Proliferation
MH  - Humans
MH  - Insulin Receptor Substrate Proteins/*metabolism
MH  - MCF-7 Cells
MH  - Polyribosomes/*metabolism
MH  - Protein Binding
MH  - Ribonucleoproteins/*metabolism
OTO - NOTNLM
OT  - 4E binding proteins
OT  - 4EBPs
OT  - BN-PAGE
OT  - DMEM
OT  - Dulbecco's modified Eagle's medium
OT  - EJC
OT  - FBS
OT  - HEK293
OT  - IGFs
OT  - IRSs
OT  - Insulin receptor substrate
OT  - Insulin-like growth factor-I (IGF-I)
OT  - MALDI-TOF-MS
OT  - MAPK
OT  - PABPC1
OT  - PI3K
OT  - PIC
OT  - RNA
OT  - RNase A
OT  - S6 protein kinases
OT  - S6Ks
OT  - Translation
OT  - blue native-polyacrylamide gel electrophoresis
OT  - eIF
OT  - eukaryotic initiation factor
OT  - exon junction complex
OT  - fetal bovine serum
OT  - human embryonic kidney 293
OT  - insulin receptor substrates
OT  - insulin-like growth factors
OT  - mRNA
OT  - mRNP
OT  - mTOR
OT  - mammalian target of rapamycin
OT  - matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry
OT  - messenger RNA
OT  - messenger ribonucleoprotein
OT  - mitogen-activated protein kinase
OT  - phosphatidylinositol 3-kinase
OT  - poly(A) binding protein cytoplasmic 1
OT  - ribonuclease A
OT  - ribosomal subunit protein S6
OT  - rpS6
OT  - translation preinitiation complex
EDAT- 2013/06/19 06:00
MHDA- 2013/10/23 06:00
CRDT- 2013/06/18 06:00
PHST- 2013/04/04 00:00 [received]
PHST- 2013/05/27 00:00 [revised]
PHST- 2013/05/27 00:00 [accepted]
PHST- 2013/06/18 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2013/10/23 06:00 [medline]
AID - S0014-5793(13)00450-X [pii]
AID - 10.1016/j.febslet.2013.05.066 [doi]
PST - ppublish
SO  - FEBS Lett. 2013 Aug 2;587(15):2319-24. doi: 10.1016/j.febslet.2013.05.066. Epub 
      2013 Jun 13.