PMID- 23771792
OWN - NLM
STAT- MEDLINE
DCOM- 20140506
LR  - 20211021
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 49
IP  - 8
DP  - 2013 Sep
TI  - Polarized neural stem cells derived from adult bone marrow stromal cells develop 
      a rosette-like structure.
PG  - 638-52
LID - 10.1007/s11626-013-9628-y [doi]
AB  - Bone marrow stromal cells (BMSCs) were reported to form floating aggregation of 
      cells with expression of nestin, a marker for neural stem cells (NSCs). The 
      purpose of this investigation is to evaluate the morphology and the molecular 
      markers expressed by NSCs derived from these neurospheres. The BMSCs were 
      isolated from Sprague Dawley rats and evaluated for osteogenesis, lipogenesis, 
      and expression of fibronectin, CD90, CD106, CD31, and Oct4. The BMSCs were 
      cultured with Dulbecco's modified Eagle's medium (DMEM)/F12 containing 15% fetal 
      bovine serum, then with DMEM/F12 containing 2% B27, basic fibroblast growth 
      factor, and epidermal growth factor. The cell aggregates or spheres were stained 
      with acridine orange, which showed that the neurospheres comprised aggregated 
      cells at either premitotic/postsynthetic (PS), postmitotic/presynthetic (PM) 
      phases of cell cycle, or a mixture of both. The NSCs harvested from the 
      neurospheres were polar with eccentric nucleus, and at either a PS or a PM cell 
      cycle phases, some cells at the latter phase tended to form rosette-like 
      structures. The cells were immunostained for molecular markers such as nestin, 
      neurofilament 68 (NF68), NF160, and NF200 and glial fibrillary acidic protein 
      (GFAP). Myelin basic protein (MBP), the pluripotency (Oct4, Nanog, and SOX2), and 
      the differentiation genes (NeuroD1, Tubb4, and Musashi I) were also evaluated 
      using reverse transcription polymerase chain reaction (RT-PCR). Nestin, NF68, 
      NF160, NF200, GFAP, O4, and N-cadherin were expressed in the NSCs. The percentage 
      of immunoreactive cells to nestin was significantly higher than that of the other 
      neuronal markers. MBP was not expressed in BMSCs, neurospheres, and NSCs. The 
      neurospheres were immunoreactive to GFAP. RT-PCR showed the expression of NeuroD1 
      and Musashi I. The pluripotency gene (SOX2) was expressed in NSCs. Oct4 and Nanog 
      were expressed in BMSCs, while Oct4 and SOX2 were expressed in the neurosphere. 
      This indicates that a pluripotency regularity network existed during the 
      transdifferentiation of BMSCs into NSCs. Image processing of the neurospheres 
      showed that the cells tended to form radial patterns. The conclusion of this 
      study is that the NSCs generated from the BMSC-derived neurospheres have the 
      morphology and the characteristics of neuroepithelial cells with tendency to 
      forming rosette-like structures.
FAU - Darabi, Shahram
AU  - Darabi S
AD  - Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran, shahram2005d@yahoo.com.
FAU - Tiraihi, Taki
AU  - Tiraihi T
FAU - Ruintan, Atefeh
AU  - Ruintan A
FAU - Abbaszadeh, Hojatt Allah
AU  - Abbaszadeh HA
FAU - Delshad, AliReza
AU  - Delshad A
FAU - Taheri, Taher
AU  - Taheri T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130615
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Nestin)
SB  - IM
MH  - Animals
MH  - Cell Aggregation/genetics
MH  - Cell Polarity/*genetics/physiology
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Lipogenesis/genetics
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Nestin/metabolism
MH  - Neural Stem Cells/*cytology/metabolism
MH  - Osteogenesis/genetics
MH  - Rats
EDAT- 2013/06/19 06:00
MHDA- 2014/05/07 06:00
CRDT- 2013/06/18 06:00
PHST- 2012/11/30 00:00 [received]
PHST- 2013/04/26 00:00 [accepted]
PHST- 2013/06/18 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/05/07 06:00 [medline]
AID - 10.1007/s11626-013-9628-y [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2013 Sep;49(8):638-52. doi: 
      10.1007/s11626-013-9628-y. Epub 2013 Jun 15.