PMID- 23772862
OWN - NLM
STAT- MEDLINE
DCOM- 20140127
LR  - 20130715
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 82
IP  - 2
DP  - 2013 Aug
TI  - Evaluation of a new flow cytometry crossmatch procedure for simultaneous 
      detection of cytotoxicity and antibody binding.
PG  - 125-30
LID - 10.1111/tan.12151 [doi]
AB  - In this study we have evaluated an alternative 96-well format flow cytometry 
      based (FCtox) method which enable simultaneous detection of cytotoxicity and 
      human leukocyte antigen (HLA) antibody binding. Comparable results were obtained 
      in side-by-side comparisons with conventional complement-dependent cytotoxicity 
      (CDC) and flow cytometric crossmatch (FCXM) in terms of sensitivity and 
      specificity. There was 91 and 93% agreement between results obtained by FCtox and 
      CDC for T and B cells, respectively. In addition, comparable results were 
      obtained with FCtox IgG and FCXM IgG for both T and B cells. Furthermore, 
      compared with a recently developed and highly sensitive Luminex based C1q assay 
      we obtained close to 90% method agreement with the FCtox assay. Our alternative 
      cytotoxicity and IgG binding assay which exhibit low intra-and inter-assay 
      variation will improve the workflow and speed up the pre-transplant testing and 
      also allow continuous monitoring of assay performance and proper quality 
      assurance.
CI  - (c) 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Alheim, M
AU  - Alheim M
AD  - Department of Laboratory Medicine, Division of Clinical Immunology and 
      Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden. 
      mats.alheim@karolinska.se
FAU - Paul, P K
AU  - Paul PK
FAU - Hauzenberger, D-M
AU  - Hauzenberger DM
FAU - Wikstrom, A-C
AU  - Wikstrom AC
LA  - eng
PT  - Journal Article
DEP - 20130617
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - B-Lymphocytes/cytology/immunology
MH  - Cytotoxicity Tests, Immunologic/*methods/standards
MH  - *Cytotoxicity, Immunologic
MH  - Flow Cytometry/*methods/standards
MH  - Humans
MH  - Immunoglobulin G/metabolism
MH  - Male
MH  - Observer Variation
MH  - Protein Binding
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - T-Lymphocytes/cytology/immunology
OTO - NOTNLM
OT  - complement-dependent cytotoxicity
OT  - crossmatching
OT  - flow cytometry
OT  - human leukocyte antigen antibodies
OT  - kidney transplantation
EDAT- 2013/06/19 06:00
MHDA- 2014/01/28 06:00
CRDT- 2013/06/19 06:00
PHST- 2013/01/30 00:00 [received]
PHST- 2013/05/08 00:00 [revised]
PHST- 2013/05/22 00:00 [accepted]
PHST- 2013/06/19 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/01/28 06:00 [medline]
AID - 10.1111/tan.12151 [doi]
PST - ppublish
SO  - Tissue Antigens. 2013 Aug;82(2):125-30. doi: 10.1111/tan.12151. Epub 2013 Jun 17.