PMID- 23774593
OWN - NLM
STAT- MEDLINE
DCOM- 20140220
LR  - 20240109
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 81
IP  - 9
DP  - 2013 Sep
TI  - Group B Streptococcus and Streptococcus suis capsular polysaccharides induce 
      chemokine production by dendritic cells via Toll-like receptor 2- and 
      MyD88-dependent and -independent pathways.
PG  - 3106-18
LID - 10.1128/IAI.00113-13 [doi]
AB  - Streptococcus agalactiae (also known as group B Streptococcus [GBS]) and 
      Streptococcus suis are encapsulated streptococci causing severe septicemia and 
      meningitis. Bacterial capsular polysaccharides (CPSs) are poorly immunogenic, but 
      anti-CPS antibodies are essential to the host defense against encapsulated 
      bacteria. The mechanisms underlying anti-CPS antibody responses are not fully 
      elucidated, but the biochemistry of CPSs, particularly the presence of sialic 
      acid, may have an immunosuppressive effect. We investigated the ability of highly 
      purified S. suis and GBS native (sialylated) CPSs to activate dendritic cells 
      (DCs), which are crucial actors in the initiation of humoral immunity. The 
      influence of CPS biochemistry was studied using CPSs extracted from different 
      serotypes within these two streptococcal species, as well as desialylated CPSs. 
      No interleukin-1beta (IL-1beta), IL-6, IL-12p70, tumor necrosis factor alpha (TNF-alpha), 
      or IL-10 production was observed in S. suis or GBS CPS-stimulated DCs. Moreover, 
      these CPSs exerted immunosuppressive effects on DC activation, as a diminution of 
      gamma interferon (IFN-gamma)-induced B cell-activating factor of the tumor necrosis 
      factor family (BAFF) expression was observed in CPS-pretreated cells. However, S. 
      suis and GBS CPSs induced significant production of CCL3, via partially Toll-like 
      receptor 2 (TLR2)- and myeloid differentiation factor 88 (MyD88)-dependent 
      pathways, and CCL2, via TLR-independent mechanisms. No major influence of CPS 
      biochemistry was observed on the capacity to induce chemokine production by DCs, 
      indicating that DCs respond to these CPSs in a patterned way rather than a 
      structure-dedicated manner.
FAU - Calzas, Cynthia
AU  - Calzas C
AD  - Laboratory of Immunology, Faculty of Veterinary Medicine, University of Montreal, 
      Saint-Hyacinthe, Quebec, Canada.
FAU - Goyette-Desjardins, Guillaume
AU  - Goyette-Desjardins G
FAU - Lemire, Paul
AU  - Lemire P
FAU - Gagnon, Fleur
AU  - Gagnon F
FAU - Lachance, Claude
AU  - Lachance C
FAU - Van Calsteren, Marie-Rose
AU  - Van Calsteren MR
FAU - Segura, Mariela
AU  - Segura M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130617
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (B-Cell Activating Factor)
RN  - 0 (Ccl3 protein, mouse)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Interleukins)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tnfsf13b protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - B-Cell Activating Factor/immunology/metabolism
MH  - B-Lymphocytes/immunology/metabolism
MH  - Chemokine CCL3/immunology/metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - Immunity, Humoral/immunology
MH  - Interferon-gamma/immunology/metabolism
MH  - Interleukins/immunology/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myeloid Differentiation Factor 88/*immunology/metabolism
MH  - Polysaccharides, Bacterial/*immunology/metabolism
MH  - Signal Transduction/immunology
MH  - Streptococcal Infections/*immunology/metabolism/microbiology
MH  - Streptococcus agalactiae/*immunology/metabolism
MH  - Streptococcus suis/*immunology/metabolism
MH  - Toll-Like Receptor 2/*immunology/metabolism
MH  - Tumor Necrosis Factor-alpha/immunology/metabolism
PMC - PMC3754219
EDAT- 2013/06/19 06:00
MHDA- 2014/02/22 06:00
PMCR- 2014/03/01
CRDT- 2013/06/19 06:00
PHST- 2013/06/19 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/02/22 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - IAI.00113-13 [pii]
AID - 00113-13 [pii]
AID - 10.1128/IAI.00113-13 [doi]
PST - ppublish
SO  - Infect Immun. 2013 Sep;81(9):3106-18. doi: 10.1128/IAI.00113-13. Epub 2013 Jun 
      17.