PMID- 23775737
OWN - NLM
STAT- MEDLINE
DCOM- 20140213
LR  - 20220318
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1034
DP  - 2013
TI  - Human leukocyte antigen (HLA) typing by DNA sequencing.
PG  - 161-95
LID - 10.1007/978-1-62703-493-7_9 [doi]
AB  - DNA sequencing is a powerful technique for identifying allelic variation within 
      the human leukocyte antigen (HLA) genes. Sequencing is usually focused on the 
      most polymorphic exons of the class I (HLA-A, -B, -C) and class II (HLA-DR, -DQ, 
      and -DP) genes. These exons encode the antigen recognition site, the region of 
      the HLA molecule that binds peptides and interacts with the T cell receptor for 
      antigen and natural killer cell immunoglobulin-like receptors (KIR). Sanger 
      sequencing of amplified DNA from each HLA gene from a preparation containing one 
      or two alleles yields a sequence that is used to identify the alleles by 
      comparison with a reference database.
FAU - Lazaro, Ana
AU  - Lazaro A
AD  - CW Bill Young Marrow Donor Recruitment and Research Program, Department of 
      Pediatrics, Georgetown University Medical Center, Rockville, MD, USA.
FAU - Tu, Bin
AU  - Tu B
FAU - Yang, Ruyan
AU  - Yang R
FAU - Xiao, Yi
AU  - Xiao Y
FAU - Kariyawasam, Kanthi
AU  - Kariyawasam K
FAU - Ng, Jennifer
AU  - Ng J
FAU - Hurley, Carolyn Katovich
AU  - Hurley CK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (HLA-DP Antigens)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Receptors, KIR)
RN  - 0 (Receptors, Natural Killer Cell)
SB  - IM
MH  - Alleles
MH  - Exons
MH  - Genotype
MH  - HLA-DP Antigens/genetics/isolation & purification
MH  - HLA-DQ Antigens/genetics/isolation & purification
MH  - HLA-DR Antigens/genetics/isolation & purification
MH  - Histocompatibility Antigens Class II/*classification/genetics
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Molecular Biology/*methods
MH  - Receptors, KIR/genetics/immunology
MH  - Receptors, Natural Killer Cell/classification/genetics/immunology
MH  - *Sequence Analysis, DNA
EDAT- 2013/06/19 06:00
MHDA- 2014/02/14 06:00
CRDT- 2013/06/19 06:00
PHST- 2013/06/19 06:00 [entrez]
PHST- 2013/06/19 06:00 [pubmed]
PHST- 2014/02/14 06:00 [medline]
AID - 10.1007/978-1-62703-493-7_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;1034:161-95. doi: 10.1007/978-1-62703-493-7_9.